CAJ | 학술논문

目的：评估环氧合酶-2（COX-2）抑制剂塞来昔布对胆管癌细胞株QBC939生长和凋亡的影响。方法通过western blot实验检测COX-2蛋白在胆管癌标本、胆管癌细胞株QBC939和肝内胆管上皮细胞HIBEpiC中的表达水平；通过WST-1实验检测塞来昔布对QBC939细胞生长的影响；通过裸鼠荷瘤模型评估塞来昔布在体内对QBC939细胞生长的影响；通过凋亡实验检测塞来昔布对QBC939细胞凋亡的影响。结果 COX-2蛋白在胆管癌组织中表达水平显著高于癌旁组织，在QBC939细胞中的表达水平显著高于HIBEpiC细胞；塞来昔布在体内和体外均可显著抑制QBC939细胞的生长；塞来昔布可诱导QBC939细胞发生凋亡。结论 COX-2抑制剂塞来昔布对胆管癌细胞的生长有显著抑制作用，该作用可能与其诱导细胞凋亡有关。
목적：평고배양합매-2（COX-2）억제제새래석포대담관암세포주QBC939생장화조망적영향。방법통과western blot실험검측COX-2단백재담관암표본、담관암세포주QBC939화간내담관상피세포HIBEpiC중적표체수평；통과WST-1실험검측새래석포대QBC939세포생장적영향；통과라서하류모형평고새래석포재체내대QBC939세포생장적영향；통과조망실험검측새래석포대QBC939세포조망적영향。결과 COX-2단백재담관암조직중표체수평현저고우암방조직，재QBC939세포중적표체수평현저고우HIBEpiC세포；새래석포재체내화체외균가현저억제QBC939세포적생장；새래석포가유도QBC939세포발생조망。결론 COX-2억제제새래석포대담관암세포적생장유현저억제작용，해작용가능여기유도세포조망유관。
Objective To evaluate the effects of Celecoxib,a selective cyclooxygenase-2 inhibitor,on the proliferation and apoptosis of QBC939 cells. Methods The expression of COX-2 in the tissue samples,QBC939 cells and HIBEpiC cells was examined using western blot assay. The in vitro inhibitory effects of Celecoxib on the proliferation of QBC939 cells were tested using WST-1 assay. The in vivo inhibitory effects of Celecoxib on the proliferation of QBC939 cells were evaluated on animal xenograft tumor models. In addition,the effects of Celecoxib on apoptosis of QBC939 cells were evaluated using Annexin V-FITC assay. Results The level of COX-2 expression in cholangiocarcinoma tissues was significantly higher than that in normal tissues and its expression in QBC939 cells was also significantly higher than that in HIBEpiC cells. Celecoxib significantly inhibited the proliferation of QBC939 cells in vitro and in vivo. In addition,Celecoxib induced apoptosis of QBC939 cells significantly. Conclusion Celecoxib can induce significant apoptosis on QBC939 cells which may lead to growth inhibition of QBC939 cells in vitro and in vivo.