CAJ | 학술논문

目的：探讨高糖状态对体外培养血管内皮细胞迁移、增殖能力的影响，以及其潜在可能的分子机理。方法：通过不同质量分数的葡萄糖干预血管内皮细胞后，用CCK－8评估内皮细胞增殖情况；用transwell实验检测内皮细胞的迁移能力；用Real －time PCR检测腺苷酸环化酶（adenylate cyclase，AC）mRNA的表达；用Western blotting 检测PKA、p－PKA蛋白的表达情况。结果：高质量分数葡萄糖干预血管内皮细胞后，血管内皮细胞迁移、增殖能力明显下降，同时AC mRNA表达明显下降，PKA蛋白表达无明显变化，而p－PKA蛋白表达明显下降。结论：高质量分数葡萄糖使血管内皮细胞迁移、增殖能力下降，cAMP－PKA信号通路的活化可能参与了血管内皮细胞增殖、迁移及修复功能。
목적：탐토고당상태대체외배양혈관내피세포천이、증식능력적영향，이급기잠재가능적분자궤리。방법：통과불동질량분수적포도당간예혈관내피세포후，용CCK－8평고내피세포증식정황；용transwell실험검측내피세포적천이능력；용Real －time PCR검측선감산배화매（adenylate cyclase，AC）mRNA적표체；용Western blotting 검측PKA、p－PKA단백적표체정황。결과：고질량분수포도당간예혈관내피세포후，혈관내피세포천이、증식능력명현하강，동시AC mRNA표체명현하강，PKA단백표체무명현변화，이p－PKA단백표체명현하강。결론：고질량분수포도당사혈관내피세포천이、증식능력하강，cAMP－PKA신호통로적활화가능삼여료혈관내피세포증식、천이급수복공능。
Objective:To determine the influence and its molecular mechanism of endothelial cell migration and proliferation in high glucos concentrations.Methods:The endothelial cells were treated in different glucose concentrations;and evaluated cell proliferation with CCK-8 reagent;and evaluated endothelial cell migration with transwell;and detected adenylate cyclase ( AC adenylate cyclase ) mRNA expression with fluorescence quantitative PCR;and detected PKA、p-PKA expression with Western blotting.Results:After endothelial cells were treated in high glucose concentrations , endothelial cell migration and proliferation significantly decreased ,and AC mRNA expression decreased obviously ,PKA of protein expression was not obviously changed ,and p-PKA expression decreased obviously.Conclusion:After endothelial cells were treated in high glucose concentrations , endothelial cell migration and proliferation are significantly decreased ,and cAMP-PKA activation may be involved in endothelial cell proliferation ,migration and repair functions.