CAJ | 학술논문

目的：研究消瘤平体内外对人宫颈癌HeLa细胞生长的影响,以及对凋亡相关蛋白Bcl-2、Bax、Fas和Survivin含量的影响。方法常规培养HeLa细胞,加入不同浓度的消瘤平,MTT法分别检测培养3个时段后对细胞生长的影响。 HeLa细胞接种于BALB/C-nu裸鼠,随机分为5组,阴性对照组、5-fu阳性对照组以及消瘤平3个剂量组,另设空白对照组,观察荷瘤裸鼠的一般状况。腹腔注射给药4周后,取瘤块和脾脏,称重计算抑瘤率和脾脏指数。取血,免疫组化法检测凋亡相关基因Bcl-2、Bax、Fas、Survivin蛋白的含量。结果消瘤平体内外均能抑制HeLa细胞的生长；体内可明显增加荷瘤裸鼠的脾脏指数；中、高剂量的消瘤平可促进Bax的表达,抑制Bcl-2和Survivin的表达,高剂量的消瘤平还促进 Fas的表达。结论消瘤平抗宫颈癌的作用机制与抑制HeLa细胞增殖,以及Bcl-2、Bax、Fas和Survivin基因蛋白诱导的凋亡有关。
목적：연구소류평체내외대인궁경암HeLa세포생장적영향,이급대조망상관단백Bcl-2、Bax、Fas화Survivin함량적영향。방법상규배양HeLa세포,가입불동농도적소류평,MTT법분별검측배양3개시단후대세포생장적영향。 HeLa세포접충우BALB/C-nu라서,수궤분위5조,음성대조조、5-fu양성대조조이급소류평3개제량조,령설공백대조조,관찰하류라서적일반상황。복강주사급약4주후,취류괴화비장,칭중계산억류솔화비장지수。취혈,면역조화법검측조망상관기인Bcl-2、Bax、Fas、Survivin단백적함량。결과소류평체내외균능억제HeLa세포적생장；체내가명현증가하류라서적비장지수；중、고제량적소류평가촉진Bax적표체,억제Bcl-2화Survivin적표체,고제량적소류평환촉진 Fas적표체。결론소류평항궁경암적작용궤제여억제HeLa세포증식,이급Bcl-2、Bax、Fas화Survivin기인단백유도적조망유관。
Objective To investigate the effect of Xiao liuping on growth and the apoptosis-related protein Bcl-2,Bax,Fas and survivin content of human cervical carcinoma HeLa cells. Methods Cells were cultivated in vitro, HeLa cells in culture medium in vitro were given with different concentrations of Xiao liuping,the inhibitory rate of the cells was measured by MTT assay. HeLa cells were seeded in BALB/C-nu mice,the tumor-bearing nude mice were di-vided into 5 groups randomly: control group,5-Fu group,Xiao liuping three doses groups,and the blank control group was set,the general condition of nude mice was observed. After 4 weeks,the tumor and spleen weight was determined, the tumor-inhibition rate and spleen index were calculated. The expression of apoptosis-related proteins Bcl-2,Bax Fas and Survivin in blood were determined by immunohistochemistry. Results Xiao liuping inhibited the proliferation of HeLa cells in vitro and in vivo,increased the spleen index of nude mice significantly,the medium and high doses of Xi-ao liuping promoted the expression of Bax,and inhibited the expression of Bcl-2 and Survivin,high dose of Xiao liup-ing promoted the expression of Fas. Conclusion The anti-tumor mechanism of Xiao liuping related to the inhibition of the proliferation,as well as Bcl-2,Bax,Fas and Survivin induced apoptosis.