CAJ | 학술논문

目的：通过Morris水迷宫实验,探讨七氟醚对老龄大鼠空间学习和记忆能力的影响。方法选取18月龄,450~500 g,健康雄性SD大鼠24只,随机分为4组。大鼠于麻醉前进行Morris水迷宫适应性训练3 d,于麻醉结束后1、3、7 d进行Morris水迷宫实验测定空间学习和记忆能力。结果①定位航行实验。组间比较：麻醉后1、3 d,I组、S1组逃避潜伏期较C组延长(P<0．05)；麻醉后3 d,I组、S1组总游泳距离较 C组延长(P<0．05)；麻醉后7 d,I组逃避潜伏期和总游泳距离较C组、S1组延长(P<0．05)。组内比较：与麻醉后1 d比较,麻醉后3、7 d各组逃避潜伏期和总游泳距离均延长(P<0．05,P<0．01)。②空间探索实验。 S1组、S2组的平台象限的停留时间(TP)和TP与总游泳时间的百分比(TP/T)与C组比较差异均有统计学意义(P<0．05),I组与C组比较差异有统计学意义(P<0．01)。结论低浓度七氟醚对老龄大鼠早期空间学习和记忆能力无明显影响。高浓度七氟醚、异氟醚均可引起老龄大鼠早期空间学习和记忆能力一过性下降,但这些变化均在麻醉后第7天趋于恢复,且七氟醚恢复较异氟醚快。
목적：통과Morris수미궁실험,탐토칠불미대노령대서공간학습화기억능력적영향。방법선취18월령,450~500 g,건강웅성SD대서24지,수궤분위4조。대서우마취전진행Morris수미궁괄응성훈련3 d,우마취결속후1、3、7 d진행Morris수미궁실험측정공간학습화기억능력。결과①정위항행실험。조간비교：마취후1、3 d,I조、S1조도피잠복기교C조연장(P<0．05)；마취후3 d,I조、S1조총유영거리교 C조연장(P<0．05)；마취후7 d,I조도피잠복기화총유영거리교C조、S1조연장(P<0．05)。조내비교：여마취후1 d비교,마취후3、7 d각조도피잠복기화총유영거리균연장(P<0．05,P<0．01)。②공간탐색실험。 S1조、S2조적평태상한적정류시간(TP)화TP여총유영시간적백분비(TP/T)여C조비교차이균유통계학의의(P<0．05),I조여C조비교차이유통계학의의(P<0．01)。결론저농도칠불미대노령대서조기공간학습화기억능력무명현영향。고농도칠불미、이불미균가인기노령대서조기공간학습화기억능력일과성하강,단저사변화균재마취후제7천추우회복,차칠불미회복교이불미쾌。
Objective To investigate the effects of sevoflurane on spatial learning and memory ability in senile rats via Morris water maze experiment. Methods Twenty-four healthy male SD rats aged 18 months weighing 450~500 g were randomly divided into four groups. The spatial learning and memory ability of rats performed adaptive train-ing of Morris water maze for three days before anesthesia were subsequently assessed with Morris water maze at 1,3 and 7 d after anesthesia. Results ①The navigation test. Compared with group C (1,3 d after anesthesia),the escape latency of both group I and group S1 were longer ( P<0. 05 ); The total swimming distance of group I and group S1 were longer at 3 d after anesthesia (P<0. 05). Compared with group C and group S1,both the escape latency and the total swimming distance of group I were extended (P<0. 05) at 7 d after anesthesia. The escape latency and the total swimming distance of all groups were more extended at 3 d (P<0. 05) and 7 d (P<0. 01) after anesthesia compared with the above indexes at 1 d after anesthesia. ②The spatial probe test. There were significant differences in the resi-dence time in platform quadrant ( TP) ,the percentage of TP and the total swimming time ( TP/T) between group S1/group S2 and group C (P<0. 05),and there were significant differences between group I and group C (P<0. 01). Conclusion Sevoflurane with low concentration has no obvious effect on early stage of spatial learning and memory a-bility in senile rats. Both sevoflurane and isoflurane with high concentration can cause transient decline on early stage of spatial learning and memory ability in senile rats,however,the changes tend to restore from the 7th day after anesthe-sia,moreover,sevoflurane restores faster than isoflurane.