CAJ | 학술논문

目的：探讨碳-12（12C）重离子诱导正常人疱疹病毒（EBV）－转化淋巴细胞染色体畸变的生物效应。方法以照射能量为165 MeV／u，剂量率为0．3～0．5 Gy／min，照射剂量分别为0．0、0．1、0．3、0．5、0．7、1．0和2．0 Gy的12 C重离子来辐照对数增长期正常人EBV-淋巴细胞，分别培养至照后48、72 h收获细胞（分别设为48和72 h组），收获前2h加秋水仙素，制片，计算染色体畸变细胞率、“双＋环”畸变率以及总非稳定性畸变率，并拟合剂量－效应回归方程。结果48 h组的“双＋环”畸变率在0.3～2.0 Gy约为72 h组的2倍。2组染色体畸变细胞数量均随照射剂量的增加而增加，呈剂量－效应关系（P＜0.01）。48 h组的畸变细胞率、“双＋环”畸变率以及非稳定性畸变率在0.0～2.0 Gy的剂量－效应回归方程分别为Y＝0.024＋0.708 D－0.175 D2[校正决定系数（ R2c ）＝0.963，P＜0.01]、Y＝－0.034＋0.531 D－0.128 D2（R2c ＝0.950，P＜0.01）和Y＝0.009＋0.868 D－0.089 D2（R2c ＝0.980，P＜0.01）；72 h组的上述指标分别为Y＝0.039＋0.388 D－0.084 D2（ R2c ＝0.976，P＜0.01）、Y＝－0.013＋0.247 D－0.062 D2（R2c ＝0.900，P＜0.01）和Y＝0.034＋0.490 D－0.046 D2（R2c ＝0.987，P＜0.01）。结论12C重离子诱导正常人EBV-转化淋巴细胞畸变随照射剂量增加而增加；染色体畸变在0.0～2.0 Gy存在一定剂量－效应关系，符合二次多项式回归模型。
목적：탐토탄-12（12C）중리자유도정상인포진병독（EBV）－전화림파세포염색체기변적생물효응。방법이조사능량위165 MeV／u，제량솔위0．3～0．5 Gy／min，조사제량분별위0．0、0．1、0．3、0．5、0．7、1．0화2．0 Gy적12 C중리자래복조대수증장기정상인EBV-림파세포，분별배양지조후48、72 h수획세포（분별설위48화72 h조），수획전2h가추수선소，제편，계산염색체기변세포솔、“쌍＋배”기변솔이급총비은정성기변솔，병의합제량－효응회귀방정。결과48 h조적“쌍＋배”기변솔재0.3～2.0 Gy약위72 h조적2배。2조염색체기변세포수량균수조사제량적증가이증가，정제량－효응관계（P＜0.01）。48 h조적기변세포솔、“쌍＋배”기변솔이급비은정성기변솔재0.0～2.0 Gy적제량－효응회귀방정분별위Y＝0.024＋0.708 D－0.175 D2[교정결정계수（ R2c ）＝0.963，P＜0.01]、Y＝－0.034＋0.531 D－0.128 D2（R2c ＝0.950，P＜0.01）화Y＝0.009＋0.868 D－0.089 D2（R2c ＝0.980，P＜0.01）；72 h조적상술지표분별위Y＝0.039＋0.388 D－0.084 D2（ R2c ＝0.976，P＜0.01）、Y＝－0.013＋0.247 D－0.062 D2（R2c ＝0.900，P＜0.01）화Y＝0.034＋0.490 D－0.046 D2（R2c ＝0.987，P＜0.01）。결론12C중리자유도정상인EBV-전화림파세포기변수조사제량증가이증가；염색체기변재0.0～2.0 Gy존재일정제량－효응관계，부합이차다항식회귀모형。
Objective To study the biological effect of chromosome aberrations in human Epstein-Barr virus-lymphocytes induced by heavy ion 12 C irradiation.Methods Human Epstein-Barr virus-lymphocytes in exponential phase were exposed to heavy ion 12 C at the irradiation doses of 0.0, 0.1, 0.3, 0.5, 0.7, 1.0 and 2.0 Gy respectively, with the beam energy was 165 MeV/u and the dose rate was 0.3-0.5 Gy/min.The division metaphase cells were collected after different culture times of 48 and 72 hours ( respectively as 48 h group and 72 h group) , colchicines was added to the samples 2 hours before collecting.For these assays, 300 cells were scored per dose and time-point to study the relationship of chromosome aberration cell rate , dicentric +ring aberration rate and the unstable chromosome aberration rate , and the regression equations of dose-response were matched.Results The dicentric+ring aberration rates of 0.3-2.0 Gy in 48 h group were double the number of that in 72 h group.Both in the two groups , the numbers of chromosome aberration cells were significantly increased with increasing irradiation dose ( P<0.01 ) , and showed dose-effect relationship .The regression equations of dose-effect of chromosome aberration cell rate , dicentric+ring aberration rate and the unstable chromosome aberration rate in 48 h group were Y=0.024+0.708 D-0.175 D2 [adjusted coefficient of determination (R2c)=0.963, P<0.01], Y=-0.034+0.531 D-0.128 D2(R2c =0.950, P<0.01), Y=0.009+0.868 D-0.089 D2(R2c =0.980, P<0.01).The regression equations of dose-effect of chromosome aberration cell rate , dicentric+ring aberration rate and the unstable chromosome aberration rate in 72 h group were Y=0.039+0.388 D-0.084 D2(R2c =0.976, P<0.01), Y=-0.013+0.247 D-0.062 D2(R2c =0.900, P<0.01), Y=0.034+0.490 D-0.046 D2(R2c =0.987, P<0.01). Conclusion It provides further evidence that the chromosome aberration in human Epstein -Barr virus-lymphocytes induced by heavy ion 12 C were increased with the increasing irradiation dose , and the chromosome aberrations shows dose-effect relationship at the dose range of 0.0-2.0 Gy, which is in accordance with quadratic polynomial regression model .