护理研究
護理研究
호리연구
NURSING RESEARCH
2015年
13期
1575-1577
,共3页
肠内喂养%温度%大鼠%胃缺血%再灌注%急性胃损伤
腸內餵養%溫度%大鼠%胃缺血%再灌註%急性胃損傷
장내위양%온도%대서%위결혈%재관주%급성위손상
enteral feeding%temperature%rat%gastric ischemia reperfusion%acute gastric mucosal lesion
[目的]探讨低温肠内喂养对胃缺血再灌注损伤(GI RI)大鼠影响及主要作用机制。[方法]将40只雄性 SD 大鼠随机分为肠内喂养温度10℃、24℃、32℃、40℃四组，采用夹闭腹腔动脉30 min 造成大鼠胃缺血，松开动脉夹后再灌注形成胃缺血再灌注损伤模型，手术后2 h 开始给予肠内营养(EN)，术后48 h 观察胃黏膜损伤指数，采用免疫组织化学方法检测肿瘤坏死因子α(TNFα)在胃黏膜细胞中的表达，采用 ELISA 法检测血清 TNF α含量。[结果]10℃组肠内喂养胃黏膜损伤指数明显低于24℃组(P <0.05)、32℃组(P <0.01)、40℃组(P <0.01)，胃黏膜 TNF α阳性表达低于24℃组(P <0.05)、32℃组(P <0.05)、40℃组(P <0.01)；胃黏膜损伤指数、TNF α阳性表达与肠内喂养温度均呈正相关(P <0.01)；各组大鼠血清 TNF α含量差异无统计学意义(P >0.05)。[结论]10℃肠内喂养温度对大鼠因缺血再灌注造成的急性胃黏膜损伤具有保护作用，其机制可能与低温抑制机体过度表达的炎症反应有关。
[目的]探討低溫腸內餵養對胃缺血再灌註損傷(GI RI)大鼠影響及主要作用機製。[方法]將40隻雄性 SD 大鼠隨機分為腸內餵養溫度10℃、24℃、32℃、40℃四組，採用夾閉腹腔動脈30 min 造成大鼠胃缺血，鬆開動脈夾後再灌註形成胃缺血再灌註損傷模型，手術後2 h 開始給予腸內營養(EN)，術後48 h 觀察胃黏膜損傷指數，採用免疫組織化學方法檢測腫瘤壞死因子α(TNFα)在胃黏膜細胞中的錶達，採用 ELISA 法檢測血清 TNF α含量。[結果]10℃組腸內餵養胃黏膜損傷指數明顯低于24℃組(P <0.05)、32℃組(P <0.01)、40℃組(P <0.01)，胃黏膜 TNF α暘性錶達低于24℃組(P <0.05)、32℃組(P <0.05)、40℃組(P <0.01)；胃黏膜損傷指數、TNF α暘性錶達與腸內餵養溫度均呈正相關(P <0.01)；各組大鼠血清 TNF α含量差異無統計學意義(P >0.05)。[結論]10℃腸內餵養溫度對大鼠因缺血再灌註造成的急性胃黏膜損傷具有保護作用，其機製可能與低溫抑製機體過度錶達的炎癥反應有關。
[목적]탐토저온장내위양대위결혈재관주손상(GI RI)대서영향급주요작용궤제。[방법]장40지웅성 SD 대서수궤분위장내위양온도10℃、24℃、32℃、40℃사조，채용협폐복강동맥30 min 조성대서위결혈，송개동맥협후재관주형성위결혈재관주손상모형，수술후2 h 개시급여장내영양(EN)，술후48 h 관찰위점막손상지수，채용면역조직화학방법검측종류배사인자α(TNFα)재위점막세포중적표체，채용 ELISA 법검측혈청 TNF α함량。[결과]10℃조장내위양위점막손상지수명현저우24℃조(P <0.05)、32℃조(P <0.01)、40℃조(P <0.01)，위점막 TNF α양성표체저우24℃조(P <0.05)、32℃조(P <0.05)、40℃조(P <0.01)；위점막손상지수、TNF α양성표체여장내위양온도균정정상관(P <0.01)；각조대서혈청 TNF α함량차이무통계학의의(P >0.05)。[결론]10℃장내위양온도대대서인결혈재관주조성적급성위점막손상구유보호작용，기궤제가능여저온억제궤체과도표체적염증반응유관。
Objective:To probe into the influence of low temperature enteral feeding on rats with gastric ischemia reperfusion injury (GI RI)and its main mechanisms.Methods:A total of 40 male Sprague Dawley(SD)rats were randomly divided into four groups according to enteral feeding temperature:10 ℃,24 ℃,32 ℃ and 40 ℃(n=10 per group).Clamping the celiac artery for 30 min to lead gastric ischemia and then to removed the clip to make GI RI model.At 2 h later after reperfusion,all rats received enteral feeding .When 48 h later after the reperfu-sion,gastric mucosal injury index was counted grossly.The expression of tumor necrosis factor α(TNF α)of gastric mucosal cell was detected by immunohistochemistry.And the plasma TNF a was detected with enzyme linked immunosorbent assay(ELISA).Results:The gastric mucosal injury index and positive expression of TNF αin 10 ℃ group were significantly lower than that in the other groups(P <0.05 or P <0.01).Meanwhile,those were positively correlated with enteral feeding temperature(P <0.01 ).There was no statistically difference in terms of plasma TNF α content among four groups(P >0.05).Conclusion:The results suggested that 10 ℃enteral feeding show a gastroprotective effect for the ischemia reperfusion injury in rats,The mechanism may be related to low temperature inhibiting the body’s inflammatory response over expression.
