CAJ | 학술논문

线粒体甘油?3?磷酸酰基转移酶基因（ Mitochondrial glycerol?3?phosphate acyltransferase， GPAM）的主要作用是通过催化三酰基甘油和磷脂生物合成过程，从而促进动物机体甘油三酯的生成，并通过中心和外周调节作用，对哺乳动物脂肪沉积、能量消耗、胴体重以及整体的代谢进行调控。设计特定引物对牛GPAM基因编码区进行PCR扩增并进行了克隆及测序，并假定牛GPAM基因编码序列发生E1－76－C＞T点突变，通过生物信息学的方法，对牛GPAM基因突变前后编码产物的理化性质、结构与功能进行了对比分析，通过生物信息学对比分析得出，牛GPAM基因单碱基的改变可以影响到氨基酸的编码，在一级结构、二级结构和三级结构方面存在显著差异。
선립체감유?3?린산선기전이매기인（ Mitochondrial glycerol?3?phosphate acyltransferase， GPAM）적주요작용시통과최화삼선기감유화린지생물합성과정，종이촉진동물궤체감유삼지적생성，병통과중심화외주조절작용，대포유동물지방침적、능량소모、동체중이급정체적대사진행조공。설계특정인물대우GPAM기인편마구진행PCR확증병진행료극륭급측서，병가정우GPAM기인편마서렬발생E1－76－C＞T점돌변，통과생물신식학적방법，대우GPAM기인돌변전후편마산물적이화성질、결구여공능진행료대비분석，통과생물신식학대비분석득출，우GPAM기인단감기적개변가이영향도안기산적편마，재일급결구、이급결구화삼급결구방면존재현저차이。
The main function of Mitochondrial glycerol?3?phosphate acyltransferase( GPAM) gene is to promote production of triglyceride in animal body through catalytic triacylglycero phospholipid bio?synthesis, and to regulate fat deposition, energy consumption, carcass weight and overall metabolism of mammals through central and peripheral regulating action. In this study, coding region of bovine GPAM gene was amplified via specific primers by PCR method, and was cloned and sequenced. Bioinformatics methods were used for comparatively analyzing physical and chemical properties, structure and function between mutant type and normal type, assuming that mutation occurred withE1-76-C>T site of bovine GPAM gene coding sequence. Compartive analysis of bioinformatics showed that bovine GPAM gene single nucleotide change can affect amino acid coding, and there are significant differences in primary structure, secondary structure and tertiary structure of protein.