粮食与油脂
糧食與油脂
양식여유지
CEREALS & OILS
2015年
4期
33-37
,共5页
磷脂酶%大豆油%非水化磷脂%响应面试验
燐脂酶%大豆油%非水化燐脂%響應麵試驗
린지매%대두유%비수화린지%향응면시험
Lecitase Ultra%soybean oil%nonhydratable phospholipids%response surface methodology
在单因素的基础上，选取酶浓度、酶解温度、酶解时间、pH四个因素为自变量，以脱胶后油脂中的磷含量为响应值，根据中心组合设计原理，利用Design–Expert 7.0软件设计响应面试验及对数据进行处理，优化酶解工艺，确定最佳工艺参数为：酶浓度0.045%、反应时间3.5 h、反应温度44.0℃、pH为4.8。在此条件进行磷脂酶Lecitase Ultra脱除大豆油中非水化磷脂实验，3次平行实验所得到的磷含量的平均值为6.2 mg/kg。
在單因素的基礎上，選取酶濃度、酶解溫度、酶解時間、pH四箇因素為自變量，以脫膠後油脂中的燐含量為響應值，根據中心組閤設計原理，利用Design–Expert 7.0軟件設計響應麵試驗及對數據進行處理，優化酶解工藝，確定最佳工藝參數為：酶濃度0.045%、反應時間3.5 h、反應溫度44.0℃、pH為4.8。在此條件進行燐脂酶Lecitase Ultra脫除大豆油中非水化燐脂實驗，3次平行實驗所得到的燐含量的平均值為6.2 mg/kg。
재단인소적기출상，선취매농도、매해온도、매해시간、pH사개인소위자변량，이탈효후유지중적린함량위향응치，근거중심조합설계원리，이용Design–Expert 7.0연건설계향응면시험급대수거진행처리，우화매해공예，학정최가공예삼수위：매농도0.045%、반응시간3.5 h、반응온도44.0℃、pH위4.8。재차조건진행린지매Lecitase Ultra탈제대두유중비수화린지실험，3차평행실험소득도적린함량적평균치위6.2 mg/kg。
The influence of Lecitase Ultra on removal of nonhydratable phospholipids in soybean oil was studied. On the basis of single–factor tests,enzyme concentration,reaction temperature,reaction time and the pH were selected as independent variables,and the phosphorus content in degummed oil was selected as response value. The test was designed to use the response surface methodology according to central composite design principle. The software Expert Design 7.0 Trial was used for data processing. The results showed that the optimum conditions were enzyme concentration 0.045%, reaction time 3.5 h,reaction temperature 44.0℃ and pH 4.8. According to this condition,the experiment of removing the nonhydratable phospholipids in soybean oil by Lecitase Ultra was made, and the average phosphorus content got from three parallel experiments was 6.2 mg/kg.
