CAJ | 학술논문

介绍了激光扫描共聚焦显微镜（ laser scanning confocal microscopy，LSCM）的原理和构造，分析了LSCM的优点和缺点，并以花粉为例，详细介绍了共聚焦针孔直径、光电倍增管检测器增益、激光强度、扫描速度、扫描方式和Z轴步距等重要参数设置对共聚焦成像的不同影响。探讨了正确使用LSCM的方法与技巧，如获取高质量的图像、图像保存及图像处理，以便为科技人员利用LSCM开展更多植物学与环境科学相关的研究提供参考。
개소료격광소묘공취초현미경（ laser scanning confocal microscopy，LSCM）적원리화구조，분석료LSCM적우점화결점，병이화분위례，상세개소료공취초침공직경、광전배증관검측기증익、격광강도、소묘속도、소묘방식화Z축보거등중요삼수설치대공취초성상적불동영향。탐토료정학사용LSCM적방법여기교，여획취고질량적도상、도상보존급도상처리，이편위과기인원이용LSCM개전경다식물학여배경과학상관적연구제공삼고。
The imaging principle of laser scanning confocal microscopy ( LSCM) was introduced in this paper. By taking the pollen as an example, the advantages and disadvantages of LSCM were discussed,and the effects of some important parameters including pinhole diameter,the gain of the photomultiplier tube detector, the intensity of the laser, scanning speed, scanning mode, Z?axis step on confocal imaging were introduced. In the meanwhile, the methods and techniques in LSCM well using were introduced, such as how to get high?quality images, images saving and image processing. These results will provide references for the researchers to carry out more botany and environmental science?related research by using LSCM.