CAJ | 학술논문

膜蛋白是生物蛋白家族的重要组成部分，由于膜蛋白所处的特殊的空间位置，对膜蛋白结构和功能的研究越来越多。单颗粒研究方法和冷冻断层方法是利用电子显微镜结构解析研究膜蛋白结构的两种主要方法。近些年以来，由于数据处理能力以及制样技术的迅速进步，膜蛋白的电镜结构研究已经取得了巨大的进展。本文主要对膜蛋白结构研究方法中的冷冻断层成像技术和单颗粒研究方法作了一个简单的介绍。
막단백시생물단백가족적중요조성부분，유우막단백소처적특수적공간위치，대막단백결구화공능적연구월래월다。단과립연구방법화냉동단층방법시이용전자현미경결구해석연구막단백결구적량충주요방법。근사년이래，유우수거처리능력이급제양기술적신속진보，막단백적전경결구연구이경취득료거대적진전。본문주요대막단백결구연구방법중적냉동단층성상기술화단과립연구방법작료일개간단적개소。
Membrane protein is an important component of biological protein family. Due to its special spatial location, researchers pay more and more attentions to studying its structure and function. Two kinds of 3D reconstruction methods by cryo?electron microscopy ( cryo?EM) , single?particle analysis ( SPA ) and electron tomography ( ET ) , are used to determinate the structure of membrane protein. As the capacity of date processing and the technology of sample preparation have undergone an exponential growth over the last decade, the determination of membrane protein structure by electron microscopy has made great progress. The recent research progresses on the study of membrane protein structure by using Single?particle method and Cryo?electron Tomography were reviewed in this article.