CAJ | 학술논문

目的：探讨腺病毒重组人骨形态发生蛋白-2（Ad-rhBMP-2）转染兔骨髓间充质干细胞（BMSCs）复合同种异体脱钙骨基质（DBM）的生物相容性。方法参照 Urist 方法制得兔同种异体 DBM 材料，免疫组化观察转染后 BMSCs 内 BMP-2表达情况；转染48 h 后复合同种异体 DBM 上，扫描电镜观察细胞生长、贴附情况，MTT 法检测细胞增殖情况。结果腺病毒转染48 h 后，BMSCs 能够表达 BMP-2，扫描电镜可见转染后的细胞在 DBM 上贴附良好并且大量增殖。MTT 检测结果显示，种植于DBM 上的转染后细胞增殖情况正常，与对照组比较差异无统计学意义（P ＞0．05）。结论Ad-BMP-2转染 BMSCs 与同种异体DBM 的生物相容性良好。
목적：탐토선병독중조인골형태발생단백-2（Ad-rhBMP-2）전염토골수간충질간세포（BMSCs）복합동충이체탈개골기질（DBM）적생물상용성。방법삼조 Urist 방법제득토동충이체 DBM 재료，면역조화관찰전염후 BMSCs 내 BMP-2표체정황；전염48 h 후복합동충이체 DBM 상，소묘전경관찰세포생장、첩부정황，MTT 법검측세포증식정황。결과선병독전염48 h 후，BMSCs 능구표체 BMP-2，소묘전경가견전염후적세포재 DBM 상첩부량호병차대량증식。MTT 검측결과현시，충식우DBM 상적전염후세포증식정황정상，여대조조비교차이무통계학의의（P ＞0．05）。결론Ad-BMP-2전염 BMSCs 여동충이체DBM 적생물상용성량호。
Objective To observe the biocompatibility of rabbit bone marrow mesenchymal stem cells (BMSCs)combined with allogeneic decalcified bone matrix(DBM)after transfecting adenoviral recombinant human bone morphogenetic protein-2(Ad-rhBMP-2).Methods The rabbit allogeneic DBM material was prepared according to the Ursit method.After transfecting Ad-BMP-2 on rabbit bone marrow mesenchymal stem cells,the immunohistochemical was used to detect the expression of BMP-2 in the transfected cells;after 48 h of transfection,the cells were planted on the allograft DBM,then the scanning electron microscopy was used to observe the cell growth and adhesion condition on material,and the proliferation condition of BMSCs was detected by MTT. Results After 48 h of adenoviral transfection,BMSCs could express BMP-2 successfully.The scanning electron microscopy showed that the cells after transfection adhered well and massively proliferated on DBM material.The MTT assay showed that the prolifer-ation condition of the cells after transfection planted on DBM was normal,which showed no statistically significant difference when compared with the control group (P >0.05).Conclusion The Ad-BMP-2 transfection on BMSCs is well biocompatible to allogene-ic DBM.