重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
10期
1314-1316
,共3页
肿瘤坏死因子受体相关肽和相关蛋白质类%脑缺血%再灌注损伤%细胞死亡
腫瘤壞死因子受體相關肽和相關蛋白質類%腦缺血%再灌註損傷%細胞死亡
종류배사인자수체상관태화상관단백질류%뇌결혈%재관주손상%세포사망
tumor necrosis factor receptor-associated peptides and related proteins%brain ischemia%reperfusion injury%cell death
目的：观察肿瘤坏死因子受体相关因子6（TRAF6）在大鼠脑缺血再灌注损伤中的作用。方法将40只成年健康 SD 大鼠按照随机对照的原则，分成5组，每组8只：假手术组，缺血组，缺血再灌注2 h 组（R2 h 组），缺血再灌注12 h 组（R12 h组），缺血再灌注24 h 组（R24 h 组）。构建大鼠脑缺血再灌注损伤模型，RT-PCR 和 Western blot 检测 TRAF6的表达变化。免疫组化检测定位 TRAF6蛋白。结果与假手术组比较，缺血组和 R2 h 组、R12 h 组、R24 h 组 TRAF6表达明显升高，差异有统计学意义（P ＜0．05）。TRAF6主要定位于神经元细胞细胞质。结论大脑遭受缺血再灌注损伤时，活化的 TRAF6参与脑细胞死亡。
目的：觀察腫瘤壞死因子受體相關因子6（TRAF6）在大鼠腦缺血再灌註損傷中的作用。方法將40隻成年健康 SD 大鼠按照隨機對照的原則，分成5組，每組8隻：假手術組，缺血組，缺血再灌註2 h 組（R2 h 組），缺血再灌註12 h 組（R12 h組），缺血再灌註24 h 組（R24 h 組）。構建大鼠腦缺血再灌註損傷模型，RT-PCR 和 Western blot 檢測 TRAF6的錶達變化。免疫組化檢測定位 TRAF6蛋白。結果與假手術組比較，缺血組和 R2 h 組、R12 h 組、R24 h 組 TRAF6錶達明顯升高，差異有統計學意義（P ＜0．05）。TRAF6主要定位于神經元細胞細胞質。結論大腦遭受缺血再灌註損傷時，活化的 TRAF6參與腦細胞死亡。
목적：관찰종류배사인자수체상관인자6（TRAF6）재대서뇌결혈재관주손상중적작용。방법장40지성년건강 SD 대서안조수궤대조적원칙，분성5조，매조8지：가수술조，결혈조，결혈재관주2 h 조（R2 h 조），결혈재관주12 h 조（R12 h조），결혈재관주24 h 조（R24 h 조）。구건대서뇌결혈재관주손상모형，RT-PCR 화 Western blot 검측 TRAF6적표체변화。면역조화검측정위 TRAF6단백。결과여가수술조비교，결혈조화 R2 h 조、R12 h 조、R24 h 조 TRAF6표체명현승고，차이유통계학의의（P ＜0．05）。TRAF6주요정위우신경원세포세포질。결론대뇌조수결혈재관주손상시，활화적 TRAF6삼여뇌세포사망。
Objective To investigate the potential role of tumor necrosis factor receptor-associated factor-6(TRAF6)in the rat cerebral ischema-reperfusion injury.Methods 40 healthy adult SD rats were divided into 5 groups(n=8)according to the ran-dom control principle:sham operation group,ischemia group,reperfusion 2 h group(R2 h),reperfusion 12 h group(R12 h)and reperfusion 24 h group(R24 h).The rat cerebral ischemia-reperfusion injury model was constructed.The change of TRAF6 expres-sion was examined by RT-PCR and Western-blot.Then,the immunohistochemistry was adopted to locate the TRAF6 protein.Re-sults Compared with the sham group,the expression of TRAF6 in the ischemia group and the R2 h,R12 h and R 24 h groups was obviously increased,but the difference had no statistical significance (P <0.05).TRAF6 was mainly located in the cytoplasm of neuronal cells.Conclusion Activated TRAF6 is involved in the brain cell death induced by cerebral ischemia-reperfusion.
