CAJ | 학술논문

目的：观察染料木素磺酸钠（GSS）对小鼠慢性肝损伤的保护作用，以及其对肝组织α7尼古丁受体（α7nAChR）和白细胞介素1β（IL-1β）蛋白表达的影响。方法取 SPF 级雄性昆明种小鼠60只分成5组，分别为对照组、模型组、GSS 低剂量组、GSS 高剂量组及阳性药物组，每组12只。除对照组外其余4组采用腹腔注射10％四氯化碳（CCl4），体积为0．1 mL／10 g，持续6周，制备小鼠慢性肝损伤动物模型。同时 GSS 高、低剂量组分别灌胃给予不同剂量的 GSS（0．30、0．10 mg／kg），阳性药物组给予联苯双酯2．50 mg／kg，对照组及模型组给予等体积生理盐水，连续6 周。测定小鼠血清 AST 及 ALT 活性，并计算 AST／ALT 比值；Western blot 方法检测α7nAChR、IL-1β蛋白表达水平。结果模型组小鼠血清 ALT、AST 水平较对照组升高（P ＜0．05），肝组织α7nAChR 蛋白表达水平降低（P ＜0．01），IL-1β的表达水平升高（P ＜0．05）；经 GSS 治疗后，血清 AST、ALT 水平明显低于模型组（P ＜0．05），α7nAChR 表达水平升高（P ＜0．01），IL-1β表达水平降低（P ＜0．05）。结论 GSS 可升高损伤的肝组织α7nAChR 的表达，激活胆碱能抗炎通路，从而减少致炎细胞因子的表达，可通过抑制炎症反应对抗小鼠慢性肝损伤。
목적：관찰염료목소광산납（GSS）대소서만성간손상적보호작용，이급기대간조직α7니고정수체（α7nAChR）화백세포개소1β（IL-1β）단백표체적영향。방법취 SPF 급웅성곤명충소서60지분성5조，분별위대조조、모형조、GSS 저제량조、GSS 고제량조급양성약물조，매조12지。제대조조외기여4조채용복강주사10％사록화탄（CCl4），체적위0．1 mL／10 g，지속6주，제비소서만성간손상동물모형。동시 GSS 고、저제량조분별관위급여불동제량적 GSS（0．30、0．10 mg／kg），양성약물조급여련분쌍지2．50 mg／kg，대조조급모형조급여등체적생리염수，련속6 주。측정소서혈청 AST 급 ALT 활성，병계산 AST／ALT 비치；Western blot 방법검측α7nAChR、IL-1β단백표체수평。결과모형조소서혈청 ALT、AST 수평교대조조승고（P ＜0．05），간조직α7nAChR 단백표체수평강저（P ＜0．01），IL-1β적표체수평승고（P ＜0．05）；경 GSS 치료후，혈청 AST、ALT 수평명현저우모형조（P ＜0．05），α7nAChR 표체수평승고（P ＜0．01），IL-1β표체수평강저（P ＜0．05）。결론 GSS 가승고손상적간조직α7nAChR 적표체，격활담감능항염통로，종이감소치염세포인자적표체，가통과억제염증반응대항소서만성간손상。
Objective To observe the protective effects of genistein sodium sulfonate(GSS)on mice chronic hepatic injury in-duced by carbon tetrachloride(CCl4 )and its influence on the protein expression of α7 nicotinic acetylcholine receptor(α7nAChR) and interleukin-1 beta (IL-1β)in liver tissue.Methods 60 SPF grade male Kunming mice were randomly divided into 5 groups,in-cluding the control group,model group,low and high doses GSS groups,and positive control group,12 cases in each group.Except for the control group,the other 4 groups were intra peritoneally injected by 10 % CCl4 with a volume of 0.1 mL/10 g for 6 weeks. The mice chronic liver injury was prepared.At the same time,the high and low doses DSS groups were given the different doses of GSS(0.30,0.10 mg/kg),the positive control group was given bifendate(DDB,2.5mg/kg),the control group and the model group were given the equal volume of normal saline for 6 consecutive weeks.The AST and ALT activity was detect and the ratio of ALT/AST was calculated;the Western blot method was used to detect the expression levels ofα7nAChR and IL-1βprotein in liver.Re-sults The serum levels of ALT and AST in the model group were increased obviously,and the expression level ofα7nAChR in the liver tissue was decreased,while the expression level of IL-1βwas increased;after the GSS treatment,the serum AST and ALT lev-els were significantly lower than those in the model group(P <0.05),while the expression level ofα7nAChR was increased (P <0.01)and the expression level of IL-1βwas decreased(P <0.05).Conclusion GSS might increase the expression ofα7nAChR in injured liver tissue,activates the cholinergic anti-inflammatory pathway,thus decreases the expression of inflammatory cytokines and antagonizes the mice chronic liver injury by inhibiting the inflammatory reaction.