CAJ | 학술논문

目的：探讨金钗石斛多糖对髓系白血病细胞 WT1基因表达的影响。方法用 CCK8方法检测金钗石斛多糖在3种白血病细胞中的 IC50值；3种细胞各分两组，对照组和处理组，对照组细胞保持正常生长，处理组给予金钗石斛多糖刺激。Hoechst33258染色检测两组细胞凋亡情况，用 RT-PCR 检测 WT1表达水平，Western blot 检测 WT1、P53和 BAX 蛋白表达。结果金钗石斛多糖在 3种白血病细胞中具有相近的 IC50值，HL-60、WEHI-3、K562细胞的 IC50分别为（110．71±6．49）、（104±48．50）、（96．66±5．10）mg／mL，3种细胞的 IC50值比较，差异无统计学意义（P ＞0．05）；处理组凋亡率高于对照组（P ＜0．05）；与对照组比较，处理组 WT1基因、蛋白表达水平降低（P ＜0．05），P53和 BAX 蛋白表达增高。结论金钗石斛多糖能明显降低WT1蛋白表达水平，且对白血病细胞有一定杀伤作用。
목적：탐토금차석곡다당대수계백혈병세포 WT1기인표체적영향。방법용 CCK8방법검측금차석곡다당재3충백혈병세포중적 IC50치；3충세포각분량조，대조조화처리조，대조조세포보지정상생장，처리조급여금차석곡다당자격。Hoechst33258염색검측량조세포조망정황，용 RT-PCR 검측 WT1표체수평，Western blot 검측 WT1、P53화 BAX 단백표체。결과금차석곡다당재 3충백혈병세포중구유상근적 IC50치，HL-60、WEHI-3、K562세포적 IC50분별위（110．71±6．49）、（104±48．50）、（96．66±5．10）mg／mL，3충세포적 IC50치비교，차이무통계학의의（P ＞0．05）；처리조조망솔고우대조조（P ＜0．05）；여대조조비교，처리조 WT1기인、단백표체수평강저（P ＜0．05），P53화 BAX 단백표체증고。결론금차석곡다당능명현강저WT1단백표체수평，차대백혈병세포유일정살상작용。
Objective To explore the effect of dendrobium nobile polysaccharides on the expression of the WT 1 gene in my-eloid leukemia cells.Methods The CCK8 assay was used to detect the half maximal inhibitory concentration(IC50 )of dendrobium nobile polysaccharides in 3 kinds of leukemia cells;the each kind of leukemia cells were divided into the treatment group and the control group.The cells in the control group maintained the normal growth,while which in the treatment group were given the den-drobium nobile polysaccharides stimulation.The Hoechst33258 staining was used to detect the apoptosis situation of the cells in the two groups.The WT l gene expression level was detected by the real-time PCR and the protein expression levels of WT1,53 and BAX were detect the Western blot.Results Dendrobium nobile polysaccharides had the similar IC50 values in 3 kinds of myeloid leukemia cells,which were (110.71±6.49),(104±48.50),(96.66±5.10)mg/mL respectively,the difference among them had no statistical significance (P >0.05);the apoptosis rate of the treatment group was higher than that of the control group (P <0.05);the expression levels of WT1 gene and protein in the treatment group were decreased compared with the control group(P <0.05), while the expression of P53 and BAX protein was increased.Conclusion Dendrobium nobile polysaccharides can obviously decrease the expression level of WT1 protein,and has a certain killing effect on myeloid leukemia cells.