CAJ | 학술논문

目的：分析妇女亚甲基四氢叶酸还原酶（MTHFR）C677T、A1298C 及甲硫氨酸合成酶还原酶（MTRR）A66G 基因多态性与不良孕产史的关联性。方法采用病例对照研究的方法，以549例正常妇女（对照组）及300例有不良孕产史（观察组）妇女为对象，采集口腔黏膜上皮细胞，提取基因组 DNA，采用基因测序技术，进行 MTHFR 及 MTRR 基因多态性检测。结果MTHFR 677TT 基因型在观察组的分布频率（10．00％）较对照组（3．46％）明显升高（χ2＝15．25，P ＜0．01）；MTHFR-1298CC基因型在观察组的分布频率（11．00％）较对照组（4．01％）明显升高（χ2＝15．66，P ＜0．01）；MTRR 66GG 基因型频率分布两组比较差异无统计学意义（χ2＝ 3．02，P ＝0．082）。两个基因的交互作用分析表明同时携带 MTHFR A1298C 突变位点和 MTRR A66G 突变位点增加了不良孕产发生的可能性（OR＝1．52，P ＝0．011）。结论MTHFR C677T 及 A1298C 与女性不良孕产的发生有一定的相关性。
목적：분석부녀아갑기사경협산환원매（MTHFR）C677T、A1298C 급갑류안산합성매환원매（MTRR）A66G 기인다태성여불량잉산사적관련성。방법채용병례대조연구적방법，이549례정상부녀（대조조）급300례유불량잉산사（관찰조）부녀위대상，채집구강점막상피세포，제취기인조 DNA，채용기인측서기술，진행 MTHFR 급 MTRR 기인다태성검측。결과MTHFR 677TT 기인형재관찰조적분포빈솔（10．00％）교대조조（3．46％）명현승고（χ2＝15．25，P ＜0．01）；MTHFR-1298CC기인형재관찰조적분포빈솔（11．00％）교대조조（4．01％）명현승고（χ2＝15．66，P ＜0．01）；MTRR 66GG 기인형빈솔분포량조비교차이무통계학의의（χ2＝ 3．02，P ＝0．082）。량개기인적교호작용분석표명동시휴대 MTHFR A1298C 돌변위점화 MTRR A66G 돌변위점증가료불량잉산발생적가능성（OR＝1．52，P ＝0．011）。결론MTHFR C677T 급 A1298C 여녀성불량잉산적발생유일정적상관성。
Objective To analyze the relationship between methylenetetrahydrofolate reductase (MTHFR)C677T,A1298C and methionine synthase reductase(MTRR)gene polymorphism with abnormal pregnancy history.Methods 549 normal women (control group)and 300 women with the abnormal pregnancy history(observation group)were taken as the subjects by adopting the case control research method.The oral mucosa epithelial cells were collected for extracting genomic DNA.The MTHFR and MTRR gene polymorphisms were detected by the gene sequencing method.Results The distribution frequency of MTHFR 677TT genotype in the abnormal pregnancy group was significantly increased compared with the control group (10.00% vs.3.46%,χ2 =15.25,P <0.01);the distribution frequency of MTHFR-1298CC genotype in the abnormal pregnancy group was significantly in-creased compared with the control group (11.00 vs.4.01%,χ2 =15.66,P <0.01);the distribution frequency of MTRR A66G gen-otype had no statistical difference between the two groups(χ2 =3.02,P =0.082).The interactive analysis of 2 genes indicated that simultaneous carrying the MTHFR A1298C mutation site and MTRR A66G mutation site increased the possibility of abnormal pregnancy occurrence (OR=1.52,P =0.011).Conclusion MTHFR C677T and A1298C have a certain correlation with female ab-normal pregnancy occurrence.