国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2015年
6期
410-416
,共7页
肺癌%二甲双胍%顺铂%生存素%基质金属蛋白酶-2%Ki67
肺癌%二甲雙胍%順鉑%生存素%基質金屬蛋白酶-2%Ki67
폐암%이갑쌍고%순박%생존소%기질금속단백매-2%Ki67
Lung caner%Metformin%Cisplatin%Survivin%Matrix metalloproteinase-2%Ki67
目的 研究二甲双胍联合常用的化疗药物顺铂对于人肺癌裸鼠移植瘤的抑瘤作用及对生存素(Survivin)、基质金属蛋白酶2(MMP-2)和Ki67分子表达的影响,致力于探索治疗肺癌的新的有效药物.方法 建立肺癌裸鼠移植瘤模型,将裸鼠随机分为二甲双胍组、顺铂组、二甲双胍联合顺铂组及对照组,给药42 d后,处死动物,留取肿瘤组织,免疫组织化学法及实时荧光定量PCR法检测肿瘤组织中Survivin、MMP-2和Ki67蛋白及mRNA的表达.结果 二甲双胍组、顺铂组和二甲双胍联合顺铂组抑瘤率分别为28.97％、35.34％和54.65％.与对照组比较,顺铂组及二甲双胍联合顺铂组Survivin、MMP-2和Ki67蛋白及mRNA表达水平均降低(P值均＜0.05),二甲双胍组MMP-2蛋白及mRNA表达水平降低(P值均＜0.05);与二甲双胍组、顺铂组比较,二甲双胍联合顺铂组Survivin、MMP-2和Ki67蛋白及mRNA表达水平均降低(P值均＜0.05).结论 二甲双胍组可抑制MMP-2的表达,顺铂组及二甲双胍联合顺铂组均可抑制Survivin、MMP-2和Ki67的表达,二甲双胍和顺铂联合应用可以增强抗肿瘤的疗效.
目的 研究二甲雙胍聯閤常用的化療藥物順鉑對于人肺癌裸鼠移植瘤的抑瘤作用及對生存素(Survivin)、基質金屬蛋白酶2(MMP-2)和Ki67分子錶達的影響,緻力于探索治療肺癌的新的有效藥物.方法 建立肺癌裸鼠移植瘤模型,將裸鼠隨機分為二甲雙胍組、順鉑組、二甲雙胍聯閤順鉑組及對照組,給藥42 d後,處死動物,留取腫瘤組織,免疫組織化學法及實時熒光定量PCR法檢測腫瘤組織中Survivin、MMP-2和Ki67蛋白及mRNA的錶達.結果 二甲雙胍組、順鉑組和二甲雙胍聯閤順鉑組抑瘤率分彆為28.97％、35.34％和54.65％.與對照組比較,順鉑組及二甲雙胍聯閤順鉑組Survivin、MMP-2和Ki67蛋白及mRNA錶達水平均降低(P值均＜0.05),二甲雙胍組MMP-2蛋白及mRNA錶達水平降低(P值均＜0.05);與二甲雙胍組、順鉑組比較,二甲雙胍聯閤順鉑組Survivin、MMP-2和Ki67蛋白及mRNA錶達水平均降低(P值均＜0.05).結論 二甲雙胍組可抑製MMP-2的錶達,順鉑組及二甲雙胍聯閤順鉑組均可抑製Survivin、MMP-2和Ki67的錶達,二甲雙胍和順鉑聯閤應用可以增彊抗腫瘤的療效.
목적 연구이갑쌍고연합상용적화료약물순박대우인폐암라서이식류적억류작용급대생존소(Survivin)、기질금속단백매2(MMP-2)화Ki67분자표체적영향,치력우탐색치료폐암적신적유효약물.방법 건립폐암라서이식류모형,장라서수궤분위이갑쌍고조、순박조、이갑쌍고연합순박조급대조조,급약42 d후,처사동물,류취종류조직,면역조직화학법급실시형광정량PCR법검측종류조직중Survivin、MMP-2화Ki67단백급mRNA적표체.결과 이갑쌍고조、순박조화이갑쌍고연합순박조억류솔분별위28.97％、35.34％화54.65％.여대조조비교,순박조급이갑쌍고연합순박조Survivin、MMP-2화Ki67단백급mRNA표체수평균강저(P치균＜0.05),이갑쌍고조MMP-2단백급mRNA표체수평강저(P치균＜0.05);여이갑쌍고조、순박조비교,이갑쌍고연합순박조Survivin、MMP-2화Ki67단백급mRNA표체수평균강저(P치균＜0.05).결론 이갑쌍고조가억제MMP-2적표체,순박조급이갑쌍고연합순박조균가억제Survivin、MMP-2화Ki67적표체,이갑쌍고화순박연합응용가이증강항종류적료효.
Objective This study is designed to evaluate the effects of metformin combined with cisplatin on tumor growth,expressions of Survivin,matrix metalloproteinase-2 (MMP-2),and Ki67 in lung cancer xenograft on nude mice,to potentially develop new effective drugs for the treatment of lung caner.Methods Human lung cancer xenograft study model was established.The nude mice were randomly divided into metformin-treated group,cisplatin-treated group,metformin combined with cisplatin-treated group and control group.Forty-two days after administration,all the nude mice were sacrificed and tumor tissues were sampled.The expressions of Survivin,MMP-2,and Ki67 proteins in the tumor tissues were detected by immumohistochemical method.The expressions of Survivin,MMP-2,and Ki67 mRNA in the tumor tissue were detected by florescent real-time quantitative PCR.Results The tumor inhibition rate in metformin-treated group,cisplatin-treated group,and metformin combined with cisplatin-treated group was 28.97％,35.34％,and 54.65％,respectively.The expressions of Survivin,MMP-2,and Ki67 protein and mRNA in cisplatin-treated group and metformin combined with cisplatintreated group were lower than those in control group (all P ＜0.05).Compared with control group,the expressions of MMP-2 protein and mRNA in metformin-treated group were reduced (all P ＜ 0.05).Compared with metformin-treated group and cisplatin-treated group,the expressions of Survivin,MMP-2,and Ki67 protein and mRNA in metformin combined with cisplatin-treated group were reduced (all P ＜ 0.05).Conclusions Metformin could inhibit MMP-2 expression.Cisplatin or metformin combined with cisplatin can inhibit the expressions of Survivin,MMP-2,and Ki67.Combination drug of metformin and cisplatin can enhance the anti-tumor efficacy.
