CAJ | 학술논문

目的：研究解旋酶DDX41在人牙髓组织和细胞中的表达，为深入探讨其在龋病及牙髓病天然免疫应答过程中的作用奠定基础。方法应用逆转录-聚合酶链反应（RT-PCR）、免疫细胞化学和免疫组织化学技术对人牙髓组织和牙髓细胞中DDX41的表达和定位进行分析。结果 RT-PCR结果显示DDX41 mRNA在人牙髓细胞中有显著表达；免疫细胞化学和免疫组织化学染色结果显示DDX41在牙髓细胞的胞浆和胞核中皆有表达，但胞核中的表达水平较低；在牙髓组织中，DDX41主要在牙髓组织表面的成牙本质细胞的胞浆和胞核中皆有表达。结论人牙髓组织中的成牙本质细胞作为牙髓免疫的效应细胞之一，在龋病和牙髓炎的免疫反应过程中，细胞内可能存在DDX41/STING依赖的TBK1-IRF3-IFN-β信号通路的活化。
목적：연구해선매DDX41재인아수조직화세포중적표체，위심입탐토기재우병급아수병천연면역응답과정중적작용전정기출。방법응용역전록-취합매련반응（RT-PCR）、면역세포화학화면역조직화학기술대인아수조직화아수세포중DDX41적표체화정위진행분석。결과 RT-PCR결과현시DDX41 mRNA재인아수세포중유현저표체；면역세포화학화면역조직화학염색결과현시DDX41재아수세포적포장화포핵중개유표체，단포핵중적표체수평교저；재아수조직중，DDX41주요재아수조직표면적성아본질세포적포장화포핵중개유표체。결론인아수조직중적성아본질세포작위아수면역적효응세포지일，재우병화아수염적면역반응과정중，세포내가능존재DDX41/STING의뢰적TBK1-IRF3-IFN-β신호통로적활화。
Objective To detect the expression of D-E-A-D-box polypeptide 41 (DDX41) in human dental pulp tissues and cells. Methods The mRNA and protein expressions of DDX41 in human dental pulp cells were detected by RT-PCR and immunocytochemistry, and the expression of DDX41 in human dental pulp tissues was investigated by immunohistochemistry. Results Strong expressions of DDX41 mRNA and protein were detected in dental pulp cells. In dental pulp tissues, DDX41 was expressed in the cytoplasm and nucleus of odontoblasts. Conclusion DDX41/STING-dependent TBK1-IRF3-IFN-βsignaling pathway may play a role in innate immune responses of the dental pulp to caries and pulpitis.