CAJ | 학술논문

该文从脱脂大豆粉中分离纯化28K（Gly m Bd 28K）蛋白，并制备多抗血清。方法用碱溶酸提法提取28K蛋白，盐析法制得28K粗蛋白，DEAE–Sepharose CL–6B层析纯化28K蛋白， SDS–聚丙烯酰胺凝胶电泳检测蛋白的纯度，制备兔抗血清，用间接ELISA法检测效价。该实验纯化出28K蛋白，制备了抗28K的多抗血清，效价为1∶51200。为研究大豆主要过敏原蛋白28K的单克隆抗体制备及28K的IgG结合表位的确定奠定了基础。
해문종탈지대두분중분리순화28K（Gly m Bd 28K）단백，병제비다항혈청。방법용감용산제법제취28K단백，염석법제득28K조단백，DEAE–Sepharose CL–6B층석순화28K단백， SDS–취병희선알응효전영검측단백적순도，제비토항혈청，용간접ELISA법검측효개。해실험순화출28K단백，제비료항28K적다항혈청，효개위1∶51200。위연구대두주요과민원단백28K적단극륭항체제비급28K적IgG결합표위적학정전정료기출。
The 28K(Gly m Bd 28K)was isolated and purified from defatted soybean flour. Then it was used to prepare the rabbit polyclonal antisera. 28K protein was extracted with an alkali solution and acid formulation. 28K crude protein was attended by ammonium sulfate precipitation method, and then DEAE–Sepharose CL–6B further purified 28K,28K was detected purity by SDS–PAGE electrophoresis,prepared the rabbit polyclonal antiserum,tested antisera titer by indirect ELISA. In the study,28K was successfully purified,the titer of rabbit antisera obtained was up to 1∶51200,and rabbit antisera were prepared. That might provide a basis for the study on major allergens of soybean protein 28K monoclonal antibody and 28K IgG determine.