CAJ | 학술논문

抗体是获得性免疫系统的主要组分，在防御性及致病性免疫反应中起着关键作用。对抗体发生、发展和成熟过程的解析，对研究传染性疾病致病机制、疫苗设计、自免疫疾病发生及进展等有着重要意义。随着高通量测序技术的发展和成熟，抗体研究进入了组学时代。通过对艾滋病患者外周血抗体谱的高通量测序及后续大数据分析挖掘已经在HIV-1广谱抗体研究中取得了丰硕的成果。随着测序技术的进一步发展、测序长度的增加及精度的提高、实验体系的优化、分析流程标准的建立及单细胞抗体测序技术的应用，抗体组学研究前景广阔。本文就抗体组学的技术、发展、应用及问题作简要综述。
항체시획득성면역계통적주요조분，재방어성급치병성면역반응중기착관건작용。대항체발생、발전화성숙과정적해석，대연구전염성질병치병궤제、역묘설계、자면역질병발생급진전등유착중요의의。수착고통량측서기술적발전화성숙，항체연구진입료조학시대。통과대애자병환자외주혈항체보적고통량측서급후속대수거분석알굴이경재HIV-1엄보항체연구중취득료봉석적성과。수착측서기술적진일보발전、측서장도적증가급정도적제고、실험체계적우화、분석류정표준적건립급단세포항체측서기술적응용，항체조학연구전경엄활。본문취항체조학적기술、발전、응용급문제작간요종술。
Antibody, the major effector in adaptive immunity, plays key roles in protective and pathogenic immune responses. Integrative analyses of antibody development, differentiation, and maturation promote the research in immune mechanism, vaccine design, and therapies for autoimmune disorders. The development of next generation sequencing technologies has enabled large-scale characterization of functional antibody repertoires. With the advantages of next generation sequencing, antibody and antibody repertoire analysis have been successfully used in identification of HIV-1-broadly neutralizing antibodies, design of rationale structure-based vaccine, and development of immunology. With increasing sequence length and precision, improvement of experimental protocols and bioinformatics analyses, and development of single cell sequencing technology, antibody repertoire sequencing will expedite the research in antibody-related immune response, and thus facilitates vaccine design for infectious diseases, clinical diagnosis and interference of autoimmune diseases. This review introduces the technologies, progresses, applications, and caveats of antibody repertoire sequencing.