中华老年多器官疾病杂志
中華老年多器官疾病雜誌
중화노년다기관질병잡지
CHINESE JOURNAL OF MULTIPLE ORGAN DISEASES IN THE ELDERLY
2015年
4期
276-281
,共6页
休克,脓毒性%细菌脂蛋白耐受%心功能%保护
休剋,膿毒性%細菌脂蛋白耐受%心功能%保護
휴극,농독성%세균지단백내수%심공능%보호
shock,septic%bacterial lipoprotein tolerance%cardiac function%protection
目的：探讨细菌脂蛋白（BLP）耐受对老龄小鼠心肌细胞的保护作用，并比较其对老龄小鼠与成年小鼠心功能影响的差异。方法选取86只健康雄性C57BL/6老龄小鼠（SPF级，24月龄）和55只成年雄性C57BL/6小鼠（6～8周龄），用小剂量BLP预处理老龄小鼠及成年小鼠诱导BLP耐受，分别比较正常对照组、假手术（sham）组、脓毒症（剖腹手术行盲肠结扎穿孔致脓毒症，CLP）组、BLP耐受＋CLP组，其中成年小鼠在盲肠结扎穿孔0，2，6，12h四个时间点做小鼠二维超声心动图，老龄小鼠在0，3，6，12h四个时间点做小鼠二维超声心动图，选择左心室短轴缩短率（FS）、射血分数（EF）、左心室舒张末期内径（LVIDd）等做为观测指标。结果老龄小鼠CLP组与BLP耐受＋CLP组的FS、EF、LVIDd自手术后均呈下降趋势，但是BLP耐受＋CLP组的降幅较CLP组小。成年小鼠BLP耐受＋CLP组0～6h时段FS、EF、LVIDd的变化趋势与CLP组相同，但各时间点数值均低于CLP组；而6h后BLP耐受＋CLP组的3项指标转而上升，至12h时高于CLP组，其中FS、EF差异均有统计学意义（P＜0.05）。BLP耐受＋CLP组老龄小鼠的FS呈现出进行性下降趋势，而在成年小鼠中6h后为进行性增高趋势，12h明显高于假手术组和CLP组（P＜0.05）。成年小鼠CLP组在术后EF上升，6h后出现下降的趋势，12h低于术前水平；而老龄小鼠CLP组术后EF进行性下降。成年小鼠BLP耐受＋CLP组术后出现的EF值呈逐渐上升趋势，相反，老龄组则进行性下降。老龄小鼠BLP耐受+CLP组的LVIDd术后进行性下降，而成年小鼠BLP耐受＋CLP组术后2h时低于术前水平（P＜0.05），6h后逐渐回升。结论脓毒症对FS、EF、LVIDd有抑制作用，老龄小鼠心脏收缩功能和舒张功能所受抑制更加严重；BLP耐受对心功能有一定的保护作用，此作用在成年小鼠较明显，在老龄小鼠未见明显保护作用。
目的：探討細菌脂蛋白（BLP）耐受對老齡小鼠心肌細胞的保護作用，併比較其對老齡小鼠與成年小鼠心功能影響的差異。方法選取86隻健康雄性C57BL/6老齡小鼠（SPF級，24月齡）和55隻成年雄性C57BL/6小鼠（6～8週齡），用小劑量BLP預處理老齡小鼠及成年小鼠誘導BLP耐受，分彆比較正常對照組、假手術（sham）組、膿毒癥（剖腹手術行盲腸結扎穿孔緻膿毒癥，CLP）組、BLP耐受＋CLP組，其中成年小鼠在盲腸結扎穿孔0，2，6，12h四箇時間點做小鼠二維超聲心動圖，老齡小鼠在0，3，6，12h四箇時間點做小鼠二維超聲心動圖，選擇左心室短軸縮短率（FS）、射血分數（EF）、左心室舒張末期內徑（LVIDd）等做為觀測指標。結果老齡小鼠CLP組與BLP耐受＋CLP組的FS、EF、LVIDd自手術後均呈下降趨勢，但是BLP耐受＋CLP組的降幅較CLP組小。成年小鼠BLP耐受＋CLP組0～6h時段FS、EF、LVIDd的變化趨勢與CLP組相同，但各時間點數值均低于CLP組；而6h後BLP耐受＋CLP組的3項指標轉而上升，至12h時高于CLP組，其中FS、EF差異均有統計學意義（P＜0.05）。BLP耐受＋CLP組老齡小鼠的FS呈現齣進行性下降趨勢，而在成年小鼠中6h後為進行性增高趨勢，12h明顯高于假手術組和CLP組（P＜0.05）。成年小鼠CLP組在術後EF上升，6h後齣現下降的趨勢，12h低于術前水平；而老齡小鼠CLP組術後EF進行性下降。成年小鼠BLP耐受＋CLP組術後齣現的EF值呈逐漸上升趨勢，相反，老齡組則進行性下降。老齡小鼠BLP耐受+CLP組的LVIDd術後進行性下降，而成年小鼠BLP耐受＋CLP組術後2h時低于術前水平（P＜0.05），6h後逐漸迴升。結論膿毒癥對FS、EF、LVIDd有抑製作用，老齡小鼠心髒收縮功能和舒張功能所受抑製更加嚴重；BLP耐受對心功能有一定的保護作用，此作用在成年小鼠較明顯，在老齡小鼠未見明顯保護作用。
목적：탐토세균지단백（BLP）내수대노령소서심기세포적보호작용，병비교기대노령소서여성년소서심공능영향적차이。방법선취86지건강웅성C57BL/6노령소서（SPF급，24월령）화55지성년웅성C57BL/6소서（6～8주령），용소제량BLP예처리노령소서급성년소서유도BLP내수，분별비교정상대조조、가수술（sham）조、농독증（부복수술행맹장결찰천공치농독증，CLP）조、BLP내수＋CLP조，기중성년소서재맹장결찰천공0，2，6，12h사개시간점주소서이유초성심동도，노령소서재0，3，6，12h사개시간점주소서이유초성심동도，선택좌심실단축축단솔（FS）、사혈분수（EF）、좌심실서장말기내경（LVIDd）등주위관측지표。결과노령소서CLP조여BLP내수＋CLP조적FS、EF、LVIDd자수술후균정하강추세，단시BLP내수＋CLP조적강폭교CLP조소。성년소서BLP내수＋CLP조0～6h시단FS、EF、LVIDd적변화추세여CLP조상동，단각시간점수치균저우CLP조；이6h후BLP내수＋CLP조적3항지표전이상승，지12h시고우CLP조，기중FS、EF차이균유통계학의의（P＜0.05）。BLP내수＋CLP조노령소서적FS정현출진행성하강추세，이재성년소서중6h후위진행성증고추세，12h명현고우가수술조화CLP조（P＜0.05）。성년소서CLP조재술후EF상승，6h후출현하강적추세，12h저우술전수평；이노령소서CLP조술후EF진행성하강。성년소서BLP내수＋CLP조술후출현적EF치정축점상승추세，상반，노령조칙진행성하강。노령소서BLP내수+CLP조적LVIDd술후진행성하강，이성년소서BLP내수＋CLP조술후2h시저우술전수평（P＜0.05），6h후축점회승。결론농독증대FS、EF、LVIDd유억제작용，노령소서심장수축공능화서장공능소수억제경가엄중；BLP내수대심공능유일정적보호작용，차작용재성년소서교명현，재노령소서미견명현보호작용。
Objective To determine the protective effect of bacterial lipoprotein (BLP) tolerance on myocardiocytes in aged mice, and compare its effect on cardiac function between aged and adult mice. Methods A total of 86 healthy male aged C57BL/6 mice (SPF grade, 24 months old) and another 55 healthy male adult C57BL/6 mice (SPF grade, 6 to 8 weeks old) were employed in this study. Low-dose BLP was used to pretreat the aged and adult mice to induce BLP tolerance. Then the mice were respectively divided into control, sham operation group (sham), sepsis group (CLP, inflicted by cecal ligation and puncture) and BLP tolerance+CLP group. Echocardiography was carried out in adult mice in 0, 2, 6 and 12h after CLP to measure left ventricle shortening fraction (FS), ejection fraction (EF), left ventricular internal diameter at end-diastole (LVIDd) in adult mice, and in aged mice at time points of 0, 3, 6 and 12h for above-mentioned indices. Results The FS, EF and LVIDd showed a downward trend after operation in the sepsis group and BLP tolerance+CLP group in aged mice, with the stronger decreases in the former than in the latter group. In the time from 0 to 6h, the BLP tolerance+CLP group in adult mice had similar tendencies in the FS, EF and LVIDd with sepsis group, but lower than the later. Then the 3 indices were increased in the BLP tolerance+CLP group in 6 h after CLP, and became higher than those of sepsis group in 12h, with FS and EF having significant differences (P<0.05). The FS in the BLP tolerance+CLP aged mice presented a progressive decline tendency, but it was in a progressive increase trend after 6 h in adult mice, and was significantly higher than those of the sham and CLP groups at 12h (P<0.05). The EF of CLP group in adult mice increased after operation, then decreased from 6h and became lower than the preoperative level at 12h. While the EF of CLP group in aged mice showed a progressive decline tendency after surgery. The EF of BLP tolerance+CLP group in adult mice was in a progressive increase trend; on the contrary, the corresponding group in aged mice was tending downwards. The LVIDd of the BLP tolerance+CLP group in aged mice was progressively reduced, while that of corresponding group in adult mice was below the preoperative level in 2h after operation (P<0.05), then gradually recovered in 6h. Conclusion Sepsis has inhibitory effects on FS, EF and LVIDd, and the effects on cardiac systolic and diastolic functions are stronger in aged mice. BLP tolerance exerts protective effect on cardiac function. This protection is apparently effective in adult mice than in aged mice.
