CAJ | 학술논문

目的：利用单一荧光试剂吖啶橙（ acridine orange， AO）和简单的单激光流式细胞仪（ FCM）计数骨髓嗜多染红细胞的微核率，达到了解化合物诱导微核作用的目的。方法分别以环磷酰胺（ CP）处理雄性KM小鼠和SD大鼠，采用AO荧光染色和单激光流式细胞仪检测小鼠和大鼠骨髓含微核的嗜多染红细胞（ MNPCE）及含微核的成熟红细胞（ MNNCE）的微核率，并对结果进行比较分析。结果随着CP浓度的增高，骨髓中MNPCE和MNNCE也相应增多，呈良好的量效关系。实验结果同时和手工计数比较，没有显著差异。结论 AO＿FCM 自动化检测方法完全适用于小鼠和大鼠骨髓MNPCE及MNNCE微核率的检测。
목적：이용단일형광시제아정등（ acridine orange， AO）화간단적단격광류식세포의（ FCM）계수골수기다염홍세포적미핵솔，체도료해화합물유도미핵작용적목적。방법분별이배린선알（ CP）처리웅성KM소서화SD대서，채용AO형광염색화단격광류식세포의검측소서화대서골수함미핵적기다염홍세포（ MNPCE）급함미핵적성숙홍세포（ MNNCE）적미핵솔，병대결과진행비교분석。결과수착CP농도적증고，골수중MNPCE화MNNCE야상응증다，정량호적량효관계。실험결과동시화수공계수비교，몰유현저차이。결론 AO＿FCM 자동화검측방법완전괄용우소서화대서골수MNPCE급MNNCE미핵솔적검측。
Objective To discriminate whether chemical compounds are micronuclei-inducing by counting the ratio of bone marrow micronucleus polychromatic erythrocytes ( MNPCE) dyed with a single fluorescence reagent ( acriding organe, AO) by single-laser flow cytometry.Methods Treating male KM mice and SD rat with cyclophosphamide ( CP) respectively, and counting their frequencies of micronucleated polychromatic erythrocytes ( MNPCE) as well as frequencies of micronucleated normochromatic erythrocytes ( MNNCE ) in bone marrow by AO and a single_laser flow cytometer (FCM), comparing and analyzing the results from different methods.Results The results showed that, along with increasing dose of CP, the ratio of MNPCE also corresponding increase, suggesting significant quantative-efficiency correlation.MNPCE were also counted manually by fluorescence microscopy, and the results showed no significant difference with that by flow cytometry.Conclusions AO_FCM fully automated detection method for the detection of MNPCE and MNNCE in mice and rat bone marrow micronucleus rate is reliable.