CAJ | 학술논문

目的：扩增西藏小型猪的肌肉生长抑制素（ MSTN）基因编码区全长序列，并对序列进行生物信息学分析。方法提取西藏小型猪肝脏组织的RNA，反转录成为cDNA，利用RT-PCR方法扩增MSTN基因编码区全长序列，将纯化的片段与pMD19-T载体连接，转化大肠杆菌DH5α，筛选阳性克隆并测定其序列，利用生物信息学方法分析其序列特征，并与其他12种物种构建系统进化树。结果西藏小型猪MSTN基因编码区全长1128 bp，编码375个氨基酸，氨基酸序列同源性分析表明，与版纳微型猪MSTN氨基酸序列相似性最高，为99％。结论 MSTN基因西藏小型猪除了与鸡和斑马鱼亲缘关系较远外，与人、犬、版纳微型猪、绵羊、山羊、牛、马、黑猩猩、大鼠、小鼠的关系都较为接近，其中与版纳微型猪的亲缘关系最为接近。
목적：확증서장소형저적기육생장억제소（ MSTN）기인편마구전장서렬，병대서렬진행생물신식학분석。방법제취서장소형저간장조직적RNA，반전록성위cDNA，이용RT-PCR방법확증MSTN기인편마구전장서렬，장순화적편단여pMD19-T재체련접，전화대장간균DH5α，사선양성극륭병측정기서렬，이용생물신식학방법분석기서렬특정，병여기타12충물충구건계통진화수。결과서장소형저MSTN기인편마구전장1128 bp，편마375개안기산，안기산서렬동원성분석표명，여판납미형저MSTN안기산서렬상사성최고，위99％。결론 MSTN기인서장소형저제료여계화반마어친연관계교원외，여인、견、판납미형저、면양、산양、우、마、흑성성、대서、소서적관계도교위접근，기중여판납미형저적친연관계최위접근。
Objective To amplify the encoding full-length sequence of Tibet mini-pigs myostatin ( MSTN) gene and analysis the sequence by bioinformatics software.Method The RNA of liver tissues from Tibet mini-pig was extracted, and reversely transcribed into cDNA.The gene coding region sequence of myostatin gene was amplified through RT-PCR, and then the purified product of PCR was ligated with a pMD19-T and transferred into the bacterium DH5αfor replication.The positive clones were screened and sequenced. The sequence characters were analyzed by using bioinformatics method and phylogeny evolution tree was constructed with other twelve species.Results The coding redion of MSTN gene was 1128bp, and coded 375 amino acids.The amino acid homology analysis showed that the homology rate of amino acid sequence was 99%.Conclusions Molecular phylogeny evolution indicated that it had a close relation with human, dog, banna minipig, sheep, goat, cattle, horse, chimpanzee, rat, mouse except chick and zebrafish, and the most closely related with banna minipig.