CAJ | 학술논문

基于序列特异性核酸酶的基因组编辑技术可以在不同物种中对目标基因进行定点敲除，并可实现特定基因片段置换，基因的定点插入等基因组靶向修饰。基因组编辑是一种精准和高效的基因工程方法，近年来快速发展并得到了广泛的应用，并将改变生物技术的现状。目前，基因组编辑在不同植物，特别是农作物中的技术体系已建立，初步展示了其在植物生物技术领域的巨大潜力。介绍了不同基因组编辑系统的工作原理，并对基因组编辑技术在植物研究中的应用及成功案例进行了综述，最后对基因组编辑在植物生物技术领域所面临的机遇与挑战进行了讨论。
기우서렬특이성핵산매적기인조편집기술가이재불동물충중대목표기인진행정점고제，병가실현특정기인편단치환，기인적정점삽입등기인조파향수식。기인조편집시일충정준화고효적기인공정방법，근년래쾌속발전병득도료엄범적응용，병장개변생물기술적현상。목전，기인조편집재불동식물，특별시농작물중적기술체계이건립，초보전시료기재식물생물기술영역적거대잠력。개소료불동기인조편집계통적공작원리，병대기인조편집기술재식물연구중적응용급성공안례진행료종술，최후대기인조편집재식물생물기술영역소면림적궤우여도전진행료토론。
Genome editing based on sequence-specific nucleases could introduce targeted genome modifications, such as site-directed gene disruption, gene replacement or insertion in various species. As a preciseand efficient tool for genome engineering, genome editing has been extensively studied and widely applied during pastseveral years, and it would eventually change the current status of biotechnology. Genome editing system has now been established in plants, especially crops and already displayed its great potential in the field of plant biotechnology. In this review, we first briefly explain the working principles of different genome editing systems, and then list some examples of different applications of genome editing in plant research and breeding. Finally, we discuss the opportunities and challenges brought by genome editing to plant biotechnology.