CAJ | 학술논문

目的：以粗毛栓菌T rametes hirsuta lg‐9为出发菌株进行原生质体的U V诱变，以期获得漆酶高产菌株。方法优化粗毛栓菌T rametes hirsuta lg‐9原生质体的制备方法，并摸索该菌株紫外诱变条件。结果筛选出2株漆酶高产菌株JM‐70S3和JM‐70S8，分别是出发菌株T rametes hirsuta lg‐9漆酶产量的93．57倍和257．24倍。结论原生质体紫外诱变是粗毛拴菌获得漆酶高产菌株的有效方法。
목적：이조모전균T rametes hirsuta lg‐9위출발균주진행원생질체적U V유변，이기획득칠매고산균주。방법우화조모전균T rametes hirsuta lg‐9원생질체적제비방법，병모색해균주자외유변조건。결과사선출2주칠매고산균주JM‐70S3화JM‐70S8，분별시출발균주T rametes hirsuta lg‐9칠매산량적93．57배화257．24배。결론원생질체자외유변시조모전균획득칠매고산균주적유효방법。
Objective To obtain the high laccase -producing strains ,we used the method of UV‐mutagenesis to the Trametes hirsuta lg‐9 protoplasts .Methods We optimized the protoplast preparation method of bacteria Trametes hirsuta lg‐9 ,and groped for the strain ultraviolet mutagenesis condition .Results JM‐70S3 and JM‐70S8 were screened out ,which produced laccase 93 .57 and 257 .24 higher than the original strains .Conclusion The UV‐mutagenesis is an effective method to obtain the high laccase -producing strains of Trametes hirsuta .