CAJ | 학술논문

目的：采用高效液相色谱法测定枸骨不同部位（嫩叶、老叶、枝、青果、红果）及不同采收期枸骨叶中芦丁和槲皮素的含量。方法采用C18色谱柱（250 mm ×4．6 mm ，5μm），流动相为甲醇－0．5％磷酸水溶液（47∶53），流速为0．7 ml／min ，检测波长为356 nm ，柱温25℃，测定枸骨中芦丁和槲皮素含量。结果芦丁和槲皮素线性范围分别为0．52～5．2μg和0．34～3．4μg，相关系数 r均为0．9999，平均回收率分别为98．6％和99．2％，RSD分别为1．70％和1．91％（n＝6）。结论枸骨不同部位及不同采收期枸骨叶中芦丁和槲皮素含量有明显差异。
목적：채용고효액상색보법측정구골불동부위（눈협、로협、지、청과、홍과）급불동채수기구골협중호정화곡피소적함량。방법채용C18색보주（250 mm ×4．6 mm ，5μm），류동상위갑순－0．5％린산수용액（47∶53），류속위0．7 ml／min ，검측파장위356 nm ，주온25℃，측정구골중호정화곡피소함량。결과호정화곡피소선성범위분별위0．52～5．2μg화0．34～3．4μg，상관계수 r균위0．9999，평균회수솔분별위98．6％화99．2％，RSD분별위1．70％화1．91％（n＝6）。결론구골불동부위급불동채수기구골협중호정화곡피소함량유명현차이。
Objective Using HPLC to determin the contents of flavonoids (rutin and quercetin) in Ilex cornuta Lindl .et Paxt .and provide the scientific basics of quality control and reasonable application .Methods The con‐tents of rutin and quercetin in Ilex cornuta Lindl .et Paxt .were analyzed by C18 column(250 mm × 4 .6 mm ,5μm) using methanol -0 .5% aqueous solution of phosphoric acid (47 :53 ) as mobile phase at the flow rate of 0 .7 ml/min and UV detection was at 356 nm .Results Rutin and quercetin respectively showed good linear relation‐ships at the range of 0 .52 ~ 5 .2μg ( r=0 .9999) and 0 .34~3 .4 μg ( r=0 .9999) .The recovery rates were 98 . 6% and 99 .2% (n=6 ) while RSD 1 .70% and 1 .91% (n=6 ) .Conclusion The contents of rutin and quercetin in different parts of Ilex cornuta Lindl .et Paxt and the leaves harvesting in different months are different .