CAJ | 학술논문

蛋白质乙酰化是一种普遍存在的、可逆而且高度调控的蛋白质翻译后修饰方式，主要发生在蛋白质赖氨酸残基的ε-NH2位。乙酰化的研究历史已达50多年，目前已成为国际上蛋白质领域的研究热点。乙酰化修饰由乙酰基转移酶和去乙酰化酶共同调节，且参与了几乎所有的生物学过程，如转录、应激反应、新陈代谢以及蛋白合成与降解等。近年来，乙酰化修饰的检测技术发展迅速，从已广泛应用的质谱法到新技术如蛋白质芯片的加入，都为深入研究乙酰化提供了强有力的工具。蛋白质乙酰化应用广泛，主要在代谢疾病中发挥着重要的调控作用，而且去乙酰化酶抑制剂已经成为治疗心脏病、糖尿病和癌症等多种疾病的有潜力的试剂。围绕乙酰化的研究历程、功能、检测技术和应用进行了探讨和归纳，并在此基础上进行了展望和讨论。
단백질을선화시일충보편존재적、가역이차고도조공적단백질번역후수식방식，주요발생재단백질뢰안산잔기적ε-NH2위。을선화적연구역사이체50다년，목전이성위국제상단백질영역적연구열점。을선화수식유을선기전이매화거을선화매공동조절，차삼여료궤호소유적생물학과정，여전록、응격반응、신진대사이급단백합성여강해등。근년래，을선화수식적검측기술발전신속，종이엄범응용적질보법도신기술여단백질심편적가입，도위심입연구을선화제공료강유력적공구。단백질을선화응용엄범，주요재대사질병중발휘착중요적조공작용，이차거을선화매억제제이경성위치료심장병、당뇨병화암증등다충질병적유잠력적시제。위요을선화적연구역정、공능、검측기술화응용진행료탐토화귀납，병재차기출상진행료전망화토론。
Protein acetylation is a ubiquitous, reversible and highly regulated protein post-translational modification. It mainly occurs in?-NH2 of protein lysine residues. Protein acetylation was firstly reported about 50 years ago, and now has been the international research hotspot in the field of protein. Acetylation is catalyzed by lysine acetyltransferases and lysine deacetylases, It was involved in almost every aspects of biological processes, including transcription, stress response, central metabolism and protein synthesis and degradation, etc. In recent years, the detection technology of acetylation, which includes mass spectrometry(MS)and protein chip and so on, has developed rapidly and provides a powerful tool for advanced study of acetylation. Protein acetylation is widely used and mainly plays an important role in metabolic diseases. In addition, histone deacetylase inhibitor(HDACIs)has become the potential agent to treat a variety of diseases such as heart disease, diabetes, and cancer. This review probes and summarizes the study process, function, detecting techniques and applications of the acetylation, and also discusses the future directions as well as the prospects of protein acetylation research.