CAJ | 학술논문

目的：观察芍药软肝合剂对H22肝癌小鼠免疫功能的影响，并初步探讨芍药软肝合剂对及VEGF、PCNA表达的影响。方法采用细胞接种的方法建立小鼠H22肝癌动物模型，随机分为正常组、模型组、环磷酰胺组、中药低、中、高剂量组，观察各组动物的整体情况，行免疫组化染色实验对肿瘤组织中VEGF、PCNA表达进行检测。结果1）成功建立小鼠H22肝癌动物模型；2）芍药软肝合剂提高荷瘤小鼠机体免疫功能；3）芍药软肝合剂能降低H22肝癌小鼠肿瘤组织中VEGF、PCNA的表达水平。结论芍药软肝合剂对H22荷瘤小鼠的胸腺、脾脏有保护作用，能提高荷瘤小鼠机体免疫功能。其能抑制荷瘤小鼠中VEGF、PCNA蛋白的表达，从而抑制肿瘤生长。
목적：관찰작약연간합제대H22간암소서면역공능적영향，병초보탐토작약연간합제대급VEGF、PCNA표체적영향。방법채용세포접충적방법건립소서H22간암동물모형，수궤분위정상조、모형조、배린선알조、중약저、중、고제량조，관찰각조동물적정체정황，행면역조화염색실험대종류조직중VEGF、PCNA표체진행검측。결과1）성공건립소서H22간암동물모형；2）작약연간합제제고하류소서궤체면역공능；3）작약연간합제능강저H22간암소서종류조직중VEGF、PCNA적표체수평。결론작약연간합제대H22하류소서적흉선、비장유보호작용，능제고하류소서궤체면역공능。기능억제하류소서중VEGF、PCNA단백적표체，종이억제종류생장。
Objective:To observe the immune effec of Shaoyao Ruangan Recipe on hepatocarcinoma H22 tu?mor-bearing mice and to preliminary discuss the express influence of Shaoyao Ruangan Recipe to Proteins such as VEGF、PCNA and In order to clarify the Mechanism of Hepatocellular Carcinoma with Shaoyao Ruangan Reci?pe. Methods:Animal models of H22 hepatic cancer with mice were built according to inoculate tumor cell.Mice were randomly divided into six groups such as the normal group,the model group,CTX group,low-dose,middle-dose and high-dose group of Shaoyao Ruangan Recipe.Observe the mices'overall condition. The tumor growth in?hibition rate spleen index was determined separately. The tumor tissue were carried out by the immune dyeing experiment to determine the protein express of VEGF、PCNA in the tumor tissue. Results:1)The animal model of Hepatocarcinoma H22 Tumor-bearing Mice was set up successfully. 2)Shaoyao Ruangan Recipe could im?prove the immunity of tumor-bearing mice. 3)The middle-dose of Shaoyao Ruangan Recipe could reduce the ex?pression of VEGF and PCNA. Conclusions:Shaoyao Ruangan Recipe can protect the spleen and thymus gland of tumor-bearing mice,also has the ability to increase the immunity of tumor-bearing micec. Its anti-tumor mechanism may relate to the control of VEGF、PCNA express in tumor-bearing mice.