CAJ | 학술논문

目的：观察乌司他丁注射液联合醒脑静注射液对脑出血模型兔血清神经特异性烯醇化酶（NSE）、水通道蛋白4（AQP-4）的影响，探讨乌司他丁联合醒脑静注射液的脑保护作用。方法40只造模成功的新西兰兔随机平均分为4组，每组10只：脑出血模型组（模型组）、乌司他丁治疗组（乌司他丁组）、醒脑静注射液治疗组（醒脑静组）以及乌司他丁加醒脑静治疗组（联合组）。应用ELISA法测定各组兔造模前（0 d），造模后第1、3、5、7日动脉血中NSE、AQP-4的含量。结果造模成功后各组NSE及AQP-4水平均升高。与模型组比较，其他各组第1日NSE、AQP-4水平差异均无统计学意义（P>0.05）；各组NSE水平第3～7日均下降（P<0.05）；乌司他丁组第7日、醒脑静组第5～7日、联合组第3～7日AQP-4水平下降（P<0.05）。与联合组比较，乌司他丁组及醒脑静组第7日NSE水平较高（P<0.05）；醒脑静组AQP-4第1～7日差异均无统计学意义（P>0.05）；乌司他丁组第5日以后AQP-4水平较高（P<0.05）。结论乌司他丁联合醒脑静注射液治疗可使脑出血模型兔动脉血中NSE及AQP-4含量下降，对脑出血具有一定的脑保护作用。
목적：관찰오사타정주사액연합성뇌정주사액대뇌출혈모형토혈청신경특이성희순화매（NSE）、수통도단백4（AQP-4）적영향，탐토오사타정연합성뇌정주사액적뇌보호작용。방법40지조모성공적신서란토수궤평균분위4조，매조10지：뇌출혈모형조（모형조）、오사타정치료조（오사타정조）、성뇌정주사액치료조（성뇌정조）이급오사타정가성뇌정치료조（연합조）。응용ELISA법측정각조토조모전（0 d），조모후제1、3、5、7일동맥혈중NSE、AQP-4적함량。결과조모성공후각조NSE급AQP-4수평균승고。여모형조비교，기타각조제1일NSE、AQP-4수평차이균무통계학의의（P>0.05）；각조NSE수평제3～7일균하강（P<0.05）；오사타정조제7일、성뇌정조제5～7일、연합조제3～7일AQP-4수평하강（P<0.05）。여연합조비교，오사타정조급성뇌정조제7일NSE수평교고（P<0.05）；성뇌정조AQP-4제1～7일차이균무통계학의의（P>0.05）；오사타정조제5일이후AQP-4수평교고（P<0.05）。결론오사타정연합성뇌정주사액치료가사뇌출혈모형토동맥혈중NSE급AQP-4함량하강，대뇌출혈구유일정적뇌보호작용。
Objective:To observe the effect of ulinastatin(UTI)combined with xingnaojing(XNJ)injection on serum neuron-specific enolase(NSE) and Aquaporin-4(AQP-4) on rabbit model of incerebral hemorrhage. Methods:40 successful model of New Zealand rabbits were randomly divided into 4 groups,each group of 10:incerebral hemorrhage model group(Group M) and UTI in treatment group(Group U) and XNJ in treatment group(Group X)and UTI combined with XNJ in treatment group(Group UX). The contents of NSE and AQP-4 in arterial blood of each rabbit were checked by ELISA method at before modeling(0 d),and treatment 1st,3rd, 5th,7th day. Results:NSE and AQP-4 levels were elevated after the success of modeling. Compared with Group M,at the 1st day,the level of NSE and AQP-4 of each group was no significant statistical difference(P>0.05);At the 3rd to 7th days,NSE of each group was decreased,there was statistically significant(P<0.05);AQP-4 of Group U at 7th day and Group X at 5th to 7th days and Group UX at 3rd to 7th days were all declined,there are statistically significant(P<0.05). Compared with UX group,at the 7th day,NSE level of Group U and Group X were higher,there was statistically significant,(P<0.05);AQP-4 of Group X were no significant statistical differ?ence(P>0.05);AQP-4 of Group U was higher at 5th and 7th days,there was significant difference(P<0.05). Conclusion:UTI combined with XNJ treatment can make the incerebral hemorrhage model in rabbit arterial blood NSE and AQP-4 declined,has certain brain protective effect on incerebral hemorrhage.