CAJ | 학술논문

目的：了解肺炎支原体(MP)感染患儿MP流行株基因分型及其流行趋势变化。方法采集2012年2月至2013年12月住院MP感染患儿的鼻咽分泌物，采用基于MP P1基因的巢式PCR及多重PCR方法进行MP基因亚型的监测，并分析其流行趋势。结果在313例标本中检出P1-I型304例(97.12%)，P1-II型8例(2.56%)，V2型变异株1例(0.32%)，基因测序结果与巢式多重PCR结果相符合。结论巢式多重PCR方法能够直接有效地对MP进行P1基因分型，研究期间MP感染株以P1-I型为主，发现1例V2变异株。
목적：료해폐염지원체(MP)감염환인MP류행주기인분형급기류행추세변화。방법채집2012년2월지2013년12월주원MP감염환인적비인분비물，채용기우MP P1기인적소식PCR급다중PCR방법진행MP기인아형적감측，병분석기류행추세。결과재313례표본중검출P1-I형304례(97.12%)，P1-II형8례(2.56%)，V2형변이주1례(0.32%)，기인측서결과여소식다중PCR결과상부합。결론소식다중PCR방법능구직접유효지대MP진행P1기인분형，연구기간MP감염주이P1-I형위주，발현1례V2변이주。
ObjectiveTo analyze the genotype and variation ofMycoplasma pneumonia (MP) strains isolated from chil-dren with MP infection in Soochow area.MethodsThe nasopharyngeal secretions from hospitalized children with MP infection were collected during January 2012 and December 2013. The nested-multiplex PCR based on MPP1 gene was performed to detect the subtype ofMP gene.ResultsIn 313 samples, 304 (97.12 %) samples were classiifed as P1-I type and 8 (2.56%) sam-ples were classiifed as P1-II type and one (0.32%) was V2 variant. Gene sequencing results were consistent with nested-multiple PCR results.ConclusionsNested-multiplex PCR is a reliable method for genotyping of MPP1 gene. During the study period, P1-I type was the common genotype and only one case of V2 variant was found.