中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2015年
1期
63-67
,共5页
宋亭亭%詹江华%高伟%刘丹丹%张辉
宋亭亭%詹江華%高偉%劉丹丹%張輝
송정정%첨강화%고위%류단단%장휘
胆道闭锁%抗原,CD14%抗原,CD34%转化生长因子β1
膽道閉鎖%抗原,CD14%抗原,CD34%轉化生長因子β1
담도폐쇄%항원,CD14%항원,CD34%전화생장인자β1
Biliary atresia%Antigens,CD14%Antigens,CD34%Transforming growth factor beta1
目的 探讨肝组织细胞表面糖蛋白14(CD14),血管内皮生长因子34(CD34),转化生长因子β1(TGF-β1)在胆道闭锁(Biliary atresia,BA)肝组织中的表达及其在肝纤维化中的作用.方法 选取2013年1月至2014年1月我院BA患儿(Kasai组)、外院BA接受肝移植自体肝组织(移植组)、胆管扩张症(扩张组,作为对照组),每组15例,术中留取肝组织.进行HE及免疫组化染色检测三种蛋白在肝组织中表达及组织定位情况,分析其与肝纤维化之间的关系.应用图像分析软件(Image-Pro-Plus)分别计算CD14,TGF-β1积分光密度值(IOD),CD34阳性表达面积(Area),SPSS17.0软件统计分析三组蛋白之间表达差异及其相关性.结果 CD14表达于肝髓窦内皮细胞,成纤维细胞,炎细胞;TGF-β1表达于成纤维细胞、胆管上皮细胞、炎细胞;CD34表达于汇管区血管内皮细胞.与对照组比较,实验组三种蛋白表达增加,从肝纤维化Ⅰ级至Ⅳ级中,CD14、TGF-p1蛋白表达量呈逐渐下降趋势,CD34表达逐步增多.实验组中三种蛋白在肝组织表达差异有统计学意义(P<0.05),其中CD14与TGF-β1肝组织表达相关性显著,TGF-β1、CD34肝组织表达呈正相关(P<0.01).结论 BA肝纤维化进展期CD14,TGF-β1表达逐渐增加,参与BA肝纤维化过程,并对BA肝硬化形成起到推动作用.到移植终末期,各种细胞缺失,纤维组织增多,三组蛋白表达逐渐下降.
目的 探討肝組織細胞錶麵糖蛋白14(CD14),血管內皮生長因子34(CD34),轉化生長因子β1(TGF-β1)在膽道閉鎖(Biliary atresia,BA)肝組織中的錶達及其在肝纖維化中的作用.方法 選取2013年1月至2014年1月我院BA患兒(Kasai組)、外院BA接受肝移植自體肝組織(移植組)、膽管擴張癥(擴張組,作為對照組),每組15例,術中留取肝組織.進行HE及免疫組化染色檢測三種蛋白在肝組織中錶達及組織定位情況,分析其與肝纖維化之間的關繫.應用圖像分析軟件(Image-Pro-Plus)分彆計算CD14,TGF-β1積分光密度值(IOD),CD34暘性錶達麵積(Area),SPSS17.0軟件統計分析三組蛋白之間錶達差異及其相關性.結果 CD14錶達于肝髓竇內皮細胞,成纖維細胞,炎細胞;TGF-β1錶達于成纖維細胞、膽管上皮細胞、炎細胞;CD34錶達于彙管區血管內皮細胞.與對照組比較,實驗組三種蛋白錶達增加,從肝纖維化Ⅰ級至Ⅳ級中,CD14、TGF-p1蛋白錶達量呈逐漸下降趨勢,CD34錶達逐步增多.實驗組中三種蛋白在肝組織錶達差異有統計學意義(P<0.05),其中CD14與TGF-β1肝組織錶達相關性顯著,TGF-β1、CD34肝組織錶達呈正相關(P<0.01).結論 BA肝纖維化進展期CD14,TGF-β1錶達逐漸增加,參與BA肝纖維化過程,併對BA肝硬化形成起到推動作用.到移植終末期,各種細胞缺失,纖維組織增多,三組蛋白錶達逐漸下降.
목적 탐토간조직세포표면당단백14(CD14),혈관내피생장인자34(CD34),전화생장인자β1(TGF-β1)재담도폐쇄(Biliary atresia,BA)간조직중적표체급기재간섬유화중적작용.방법 선취2013년1월지2014년1월아원BA환인(Kasai조)、외원BA접수간이식자체간조직(이식조)、담관확장증(확장조,작위대조조),매조15례,술중류취간조직.진행HE급면역조화염색검측삼충단백재간조직중표체급조직정위정황,분석기여간섬유화지간적관계.응용도상분석연건(Image-Pro-Plus)분별계산CD14,TGF-β1적분광밀도치(IOD),CD34양성표체면적(Area),SPSS17.0연건통계분석삼조단백지간표체차이급기상관성.결과 CD14표체우간수두내피세포,성섬유세포,염세포;TGF-β1표체우성섬유세포、담관상피세포、염세포;CD34표체우회관구혈관내피세포.여대조조비교,실험조삼충단백표체증가,종간섬유화Ⅰ급지Ⅳ급중,CD14、TGF-p1단백표체량정축점하강추세,CD34표체축보증다.실험조중삼충단백재간조직표체차이유통계학의의(P<0.05),기중CD14여TGF-β1간조직표체상관성현저,TGF-β1、CD34간조직표체정정상관(P<0.01).결론 BA간섬유화진전기CD14,TGF-β1표체축점증가,삼여BA간섬유화과정,병대BA간경화형성기도추동작용.도이식종말기,각충세포결실,섬유조직증다,삼조단백표체축점하강.
Objective To explore the expression of surface glycoprotein protein hepatocyte 14 (CD14),vascular endothelial growth factor 34 (CD34) and transforming growth factor-β1 (TGF-β1) in liver biopsy of biliary atresia (BA) and examine the role of liver fibrosis.Methods From January 2013 to January 2014,liver biopsy specimens were collected intraoperatively from BA patients undergoing Kasai procedure (Kasai group),BA patients after Kasai procedure with liver transplantation (transplantation group) and congenital biliary dilation patients (CBD group as control) (n =15 each).The hematoxylin & eosin staining and immunohistochemistry were used to observe the degree of liver fibrosis and detect the expression of CD14,CD34 and TGF-β1 and determine their localizations.The image analysis software of Image-Pro-Plus was used to analyze the values of integrated optical density (IOD) for CD14 and TGF-β1 proteins in different groups and CD34 positive area.SPSS 17.0 statistical software was used for single factor ANOVA analysis (P<0.05).Pearson correlation analysis (P<0.01) showed that there was correlation between three groups about the expression of different proteins.Results CD14 was expressed in myeloid liver sinusoidal endothelial cells,fibroblasts and inflammatory cells.And TGF-31 was expressed in fibroblasts,biliary epithelial cells and inflammatory cells while CD34 in portal area and proliferating vascular endothelial cells.Compared with CBD group,the expressions of CD34,CD14 and TGF-31 proteins were up-regulated in experimental group.From the perspective of liver fibrosis grading (stage Ⅰ-Ⅳ) in BA.The expression of CD14 and TGF-β1 proteins decreased gradually while the expression of CD34 increased.The difference was statistically significant between three groups about the expression of CD14,TGF-β1 and CD34 proteins (P<0.05),the expression of CD14 and TGF-β1 proteins were significantly correlated (P<0.05) whereas the expression of TGF-β1 and CD34 proteins significantly positively correlated.Conclusions The expression of CD14 and TGF-β1 in liver fibrosis increase gradually in BA.And liver cirrhosis may be accelerated.In advanced disease,all cells are damaged and become substituted by fibrosis.And the levels of three proteins gradually decline.