CAJ | 학술논문

目的：探讨妊娠期糖尿病（gestational diabetes mellitus ， GDM）与孕妇血清、脐血和胎盘组织中核转录因子NF－κBp65的相关性。方法收集2011年11月至2013年3月天津市中心妇产科医院157名足月妊娠孕妇及正常未孕女性的临床资料，其中GDM孕妇38例（Ⅰ组）、正常孕妇56名（Ⅱ组）、正常未孕女性63名（Ⅲ组）。采用酶联免疫吸附测定3组研究对象血清、脐血中NF－κBp65的表达，采用免疫组化法检测孕妇胎盘组织中NF－κBp65的表达，并进行比较分析。结果Ⅰ组母体血清和脐血中NF－κBp65水平均高于Ⅱ组（均P＜0.05）；Ⅱ组、Ⅲ组母体血清中NF－κBp65水平比较差异无统计学意义（P＞0.05）；Ⅰ组胎盘组织中的NF－κBp65表达阳性率100.0％（38／38）高于Ⅱ组的12.5％（7／56）（ P＜0.05）。结论 NF－KBp65可能在GDM的病理生理过程中发挥了一定作用。
목적：탐토임신기당뇨병（gestational diabetes mellitus ， GDM）여잉부혈청、제혈화태반조직중핵전록인자NF－κBp65적상관성。방법수집2011년11월지2013년3월천진시중심부산과의원157명족월임신잉부급정상미잉녀성적림상자료，기중GDM잉부38례（Ⅰ조）、정상잉부56명（Ⅱ조）、정상미잉녀성63명（Ⅲ조）。채용매련면역흡부측정3조연구대상혈청、제혈중NF－κBp65적표체，채용면역조화법검측잉부태반조직중NF－κBp65적표체，병진행비교분석。결과Ⅰ조모체혈청화제혈중NF－κBp65수평균고우Ⅱ조（균P＜0.05）；Ⅱ조、Ⅲ조모체혈청중NF－κBp65수평비교차이무통계학의의（P＞0.05）；Ⅰ조태반조직중적NF－κBp65표체양성솔100.0％（38／38）고우Ⅱ조적12.5％（7／56）（ P＜0.05）。결론 NF－KBp65가능재GDM적병리생리과정중발휘료일정작용。
Objective To explore the correlation between gestational diabetes mellitus ( GDM ) and the nuclear transcription factor NF-κBp65 in maternal blood , umbilical cord blood and placental tissue .Methods Data of 157 pregnant women in The Department of Gynecology and Obstetrics Tianjin Central Hospital from Nov 2011 to Mar 2013 were collected .38 pregnant with GDM ( group Ⅰ) , 56 women with normal pregnancy ( group Ⅱ) and 63 women of childbearing age ( group Ⅲ) were enrolled .ELISA techniques was employed to measure the expression of NF -κBp65 in the blood serum and umbilical cord blood , and the expression of NF-κBp65 in placental tissue was measured by immunohistochemical staining .The results were compared between 3 groups.Results NF-kappa Bp65 level in maternal blood serum and cord blood of group Ⅰ were higher than group II (P<0.05).NF-kappa Bp65 level in maternal blood serum and cord blood between group Ⅱ and group Ⅲ had no significant difference (P>0.05).The positive expression rate of NF -kappa Bp65 in placental tissue of group Ⅰ[100.0% (38/38)] was higher than group Ⅱ[12.5%(7/56)] (P<0.05).Conclusion NF-KBp65 may play a certain role in the pathophysiology of gestational diabetes mellitus .