CAJ | 학술논문

目的：探讨人子宫旁韧带成纤维细胞在机械力作用下线粒体形态及细胞衰老水平的改变。方法选取10例武汉大学人民医院收治的因宫颈上皮内瘤变（ cervical intraepithelial neoplasia ，CIN） II－III级行阴式全子宫切除术患者的宫旁韧带（主韧带、骶韧带），进行原代培养，使用四点弯曲细胞力学加载系统对细胞进行力学加载，加力时间设定为4 h，加力大小为0、1333μstrain（1 mm）、5333μstrain（4 mm），把不加力的细胞设为对照组。采用新型透射电子显微镜HT 7700观察5000倍视野下线粒体形态变化，采用β－半乳糖苷酶染色试剂盒检测细胞衰老。结果随着机械力大小的增加，细胞的线粒体形态发生改变，线粒体出现空泡化，细胞骨架结构改变，细胞内出现凋亡小体，染色呈蓝色的细胞数量增多，细胞衰老率增加，与对照组相比，差异有统计学意义（ P＜0.05）。结论一定范围内的机械力可导致人子宫旁韧带成纤维细胞的线粒体发生损伤性改变，并促进细胞衰老。
목적：탐토인자궁방인대성섬유세포재궤계력작용하선립체형태급세포쇠로수평적개변。방법선취10례무한대학인민의원수치적인궁경상피내류변（ cervical intraepithelial neoplasia ，CIN） II－III급행음식전자궁절제술환자적궁방인대（주인대、저인대），진행원대배양，사용사점만곡세포역학가재계통대세포진행역학가재，가력시간설정위4 h，가력대소위0、1333μstrain（1 mm）、5333μstrain（4 mm），파불가력적세포설위대조조。채용신형투사전자현미경HT 7700관찰5000배시야하선립체형태변화，채용β－반유당감매염색시제합검측세포쇠로。결과수착궤계력대소적증가，세포적선립체형태발생개변，선립체출현공포화，세포골가결구개변，세포내출현조망소체，염색정람색적세포수량증다，세포쇠로솔증가，여대조조상비，차이유통계학의의（ P＜0.05）。결론일정범위내적궤계력가도치인자궁방인대성섬유세포적선립체발생손상성개변，병촉진세포쇠로。
Objective To investigate the effect of mechanical stress on the mitochondrial morphology and cell senescence of fibroblasts in human parametrial ligament .Methods The sacral ligament and cardinal ligament of 10 patients in People’ s Hospital of Wuhan University with cervical intraepithelial neoplasia ( CIN ) II -III who received total vaginal hysterectomy were selected and fibroblasts were derived with primary culture .Fibroblasts were stretched by four -point bending system with the force of 0, 1 333μstrain (1 mm), 5 333 μstrain (4 mm), using 0 μstrain as the control group, and the time fixed for 4 hours.Using the HITACHI transmission electron microscope ( HT 7 700 ) to observe the change of mitochondrial morphology under 5 000 times magnification . Usingβ-galactosidase staining kit to detect cell senescence .Results With the increase of mechanical force , the mitochondrial morphology of fibroblasts changed , the mitochondria appeared vacuolization , and cell skeleton structure damaged with the cell apoptosis body, the number of cells dyed blue and cell senescence rate increased , the difference was statistically significant compared with the control group ( P<0.05) .Conclusion Mechanical forces within a certain range can lead to the mitochondrial injury and the increase of the occurrence of cell senescence in pelvic fibroblast .