CAJ | 학술논문

目的：探讨慢性束缚应激模型对小鼠社交行为的影响，为应激所致抑郁模型的社会行为学评价方法提供数据支撑。方法采用慢性束缚加孤养方法建立应激所致抑郁模型，模型组每日束缚4 h（10：00～14：00），连续进行35 d，单笼饲养。应用糖水偏爱测试确定模型成功，应用社会趋避实验评价慢性束缚应激所致抑郁模型对小鼠社交行为的影响。结果糖水偏爱实验结果显示，慢性束缚组糖水偏爱指数明显下降，与正常组比较具有显著性差异（ P ＜0.01）。社会趋避实验结果显示，正常组对刺激鼠笼的嗅闻时间及频次多于空鼠笼，并具有显著性差异（ P ＜0.05，P ＜0.01）；慢性束缚组对刺激鼠笼和空鼠笼的嗅闻时间及频次相比，无显著性差异。慢性束缚组嗅闻刺激鼠笼时间及频次明显少于正常组，二者相比具有显著性差异（P ＜0.05，P ＜0.01）。慢性束缚组动物第一次嗅闻刺激鼠笼的潜伏期明显比正常组长，二者相比具有极显著性差异（ P ＜0.01）。慢性束缚组对刺激鼠笼的偏好指数明显小于正常组，二者具有极显著性差异（ P ＜0.01）。结论慢性束缚应激小鼠社交回避的行为学表现，为应激所致抑郁模型社会行为学评价体系的建立提供数据支撑。
목적：탐토만성속박응격모형대소서사교행위적영향，위응격소치억욱모형적사회행위학평개방법제공수거지탱。방법채용만성속박가고양방법건립응격소치억욱모형，모형조매일속박4 h（10：00～14：00），련속진행35 d，단롱사양。응용당수편애측시학정모형성공，응용사회추피실험평개만성속박응격소치억욱모형대소서사교행위적영향。결과당수편애실험결과현시，만성속박조당수편애지수명현하강，여정상조비교구유현저성차이（ P ＜0.01）。사회추피실험결과현시，정상조대자격서롱적후문시간급빈차다우공서롱，병구유현저성차이（ P ＜0.05，P ＜0.01）；만성속박조대자격서롱화공서롱적후문시간급빈차상비，무현저성차이。만성속박조후문자격서롱시간급빈차명현소우정상조，이자상비구유현저성차이（P ＜0.05，P ＜0.01）。만성속박조동물제일차후문자격서롱적잠복기명현비정상조장，이자상비구유겁현저성차이（ P ＜0.01）。만성속박조대자격서롱적편호지수명현소우정상조，이자구유겁현저성차이（ P ＜0.01）。결론만성속박응격소서사교회피적행위학표현，위응격소치억욱모형사회행위학평개체계적건립제공수거지탱。
Objective To investigate the effect of chronic restraint stress on sociability in mice.Method Chronic restraint stress was applied to establish stress depression model.The mice were individually housing and restrained for 4 h per day in a 50 mL polypropylene conical tube with ventilation holes.This daily restraint was repeated for 35 consecutive days.The sucrose preference test was applied to evaluate the model.The social approach-avoidance test was used to assess the effect of chronic restraint stress on sociability.Results The sucrose intake was significantly reduced in chronic restraint stress model compared with the control mice ( P <0.01 ) .The control group investigated the social stimulus (stranger cage)significantly longer than the object stimulus(empty cage), thereby demonstrating normal social preference.The model group showed a decrease ( sniffing time and frequency) in social investigation ( P <0.05,P <0.01, vs the control group). The chronic restraint stress group did not demonstrate social preference.The preference index in chronic restraint stress model ( ratio time sniffing stranger vs.empty cage) was significantly lower compared with the control mice (P <0.01).Conclusion The chronic restraint stress showed social avoidance in sociability test.