中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2015年
4期
368-373
,共6页
郑雅匀%曹玉玺%付士红%程璟侠%赵俊英%代培芳%孔祥盛%梁国栋
鄭雅勻%曹玉璽%付士紅%程璟俠%趙俊英%代培芳%孔祥盛%樑國棟
정아균%조옥새%부사홍%정경협%조준영%대배방%공상성%량국동
蚊媒病毒%乙型脑炎病毒%盖塔病毒%库蚊黄病毒
蚊媒病毒%乙型腦炎病毒%蓋塔病毒%庫蚊黃病毒
문매병독%을형뇌염병독%개탑병독%고문황병독
Mosquito-borne arboviruses%Japanese encephalitis virus%Getah virus%Culex flavivirus
目的 了解山西省运城市蚊虫及蚊媒病毒的种类和分布.方法 2012年8月在山西省运城市临猗县和永济市采集蚊虫标本,经鉴定分类和分批研磨后,利用细胞(C6/36和BHK21)培养方法分离病毒,对阳性分离物使用蚊媒病毒种属特异引物进行RT-PCR扩增鉴定.结果 采集4属7种10455只蚊虫,临猗县和永济市优势蚊种分别为淡色库蚊(91.96%)和三带喙库蚊(72.85%).经鉴定分析,从标本中分离到15株基因I型乙型脑炎病毒(JEV)、4株库蚊黄病毒(CxFV)、3株淡色库蚊浓核病毒(CppDNV)和1株盖塔病毒(GETV).结论 首次从山西省分离GETV和CxFV;三带喙库蚊仍是运城市优势蚊种,目前当地自然循环的JEV仍以基因I型为主.
目的 瞭解山西省運城市蚊蟲及蚊媒病毒的種類和分佈.方法 2012年8月在山西省運城市臨猗縣和永濟市採集蚊蟲標本,經鑒定分類和分批研磨後,利用細胞(C6/36和BHK21)培養方法分離病毒,對暘性分離物使用蚊媒病毒種屬特異引物進行RT-PCR擴增鑒定.結果 採集4屬7種10455隻蚊蟲,臨猗縣和永濟市優勢蚊種分彆為淡色庫蚊(91.96%)和三帶喙庫蚊(72.85%).經鑒定分析,從標本中分離到15株基因I型乙型腦炎病毒(JEV)、4株庫蚊黃病毒(CxFV)、3株淡色庫蚊濃覈病毒(CppDNV)和1株蓋塔病毒(GETV).結論 首次從山西省分離GETV和CxFV;三帶喙庫蚊仍是運城市優勢蚊種,目前噹地自然循環的JEV仍以基因I型為主.
목적 료해산서성운성시문충급문매병독적충류화분포.방법 2012년8월재산서성운성시림의현화영제시채집문충표본,경감정분류화분비연마후,이용세포(C6/36화BHK21)배양방법분리병독,대양성분리물사용문매병독충속특이인물진행RT-PCR확증감정.결과 채집4속7충10455지문충,림의현화영제시우세문충분별위담색고문(91.96%)화삼대훼고문(72.85%).경감정분석,종표본중분리도15주기인I형을형뇌염병독(JEV)、4주고문황병독(CxFV)、3주담색고문농핵병독(CppDNV)화1주개탑병독(GETV).결론 수차종산서성분리GETV화CxFV;삼대훼고문잉시운성시우세문충,목전당지자연순배적JEV잉이기인I형위주.
Objective To investigate the species and distribution of mosquitoes and mosquito-borne arboviruses in Yuncheng city of Shanxi province,China.Methods Mosquito samples were collected in 19 collection sites from Linyi county and Yongji city in Yuncheng city,in August,2012.After identification and classification,all the specimens were homogenized and centrifuged to acquire supernatant before being inoculated to both C6/36 and BHK21 cells for viral isolation.Positive isolates were identified with arbovirus species-specific primers under RT-PCR,for further sequencing and phylogenetic analysis.Results A total of 10 455 mosquitoes of 7 species in 4 genuese were collected.The predominant mosquito species in Linyi county was Culex pipens pallens (91.96%,3 911/4 253),but the one in Yongji city was Culex tritaeniorhynchus (72.85%,4 518/6 202).A total of 23 strains of viruses were isolated from the mosquito pools.15 strains from Culex tritaeniorhynchus and Culex pipens pallens were identified as genotype I Japanese encephalitis virus (JEV).Four strains from Culex pipens pallens were identified as Culex flavivirus (CxFV).Three strains from Culex pipens pallens were identified as Culex pipiens pallens densovirus (CppDNV).One strain from Armigeres subalbatus and Aedes albopictus was identified as Getah virus (GETV).Conclusion Four kinds of arboviruses were isolated from the mosquito pools,including GETV and CxFV,which were isolated and documented in Shanxi province for the first time.In the city of Yuncheng,Culex tritaeniorhynchus had been the predominant species and major vector for transmitting JEV.Genotype I JEV remained the major JEV circulating in the local natural environment.