广东微量元素科学
廣東微量元素科學
엄동미량원소과학
GUANGDONG TRACE ELEMENTS SCIENCE
2015年
2期
10-13
,共4页
缺血修饰白蛋白%钴-白蛋白结合%心肌缺血疾病
缺血脩飾白蛋白%鈷-白蛋白結閤%心肌缺血疾病
결혈수식백단백%고-백단백결합%심기결혈질병
ischemia modified albumin%albumin cobalt binding%myocardial ischemia
目的:建立改良的分光光度法测定血清缺血修饰白蛋白。方法为通过确定试剂中的各成分的含量、缓冲体系、抗干扰体系以及抗氧化体系等关键技术的解决,建立改良钴-白蛋白结合测试法测定血清IMA。结果该法测定的血清缺血修饰白蛋白,线性范围为0~180 U/mL,平均回收率为99.3%,批内变异系数和批间变异系数分别为1.17%~3.11%和2.15%~4.21%,准确性和重复性较好,线性回归方程为y=0.9867 x+0.4,相关系数为r=0.9993。结论用该文建立的方法测定血清IMA含量,具有测定简便、灵敏度高等特点,适合推广应用。
目的:建立改良的分光光度法測定血清缺血脩飾白蛋白。方法為通過確定試劑中的各成分的含量、緩遲體繫、抗榦擾體繫以及抗氧化體繫等關鍵技術的解決,建立改良鈷-白蛋白結閤測試法測定血清IMA。結果該法測定的血清缺血脩飾白蛋白,線性範圍為0~180 U/mL,平均迴收率為99.3%,批內變異繫數和批間變異繫數分彆為1.17%~3.11%和2.15%~4.21%,準確性和重複性較好,線性迴歸方程為y=0.9867 x+0.4,相關繫數為r=0.9993。結論用該文建立的方法測定血清IMA含量,具有測定簡便、靈敏度高等特點,適閤推廣應用。
목적:건립개량적분광광도법측정혈청결혈수식백단백。방법위통과학정시제중적각성분적함량、완충체계、항간우체계이급항양화체계등관건기술적해결,건립개량고-백단백결합측시법측정혈청IMA。결과해법측정적혈청결혈수식백단백,선성범위위0~180 U/mL,평균회수솔위99.3%,비내변이계수화비간변이계수분별위1.17%~3.11%화2.15%~4.21%,준학성화중복성교호,선성회귀방정위y=0.9867 x+0.4,상관계수위r=0.9993。결론용해문건립적방법측정혈청IMA함량,구유측정간편、령민도고등특점,괄합추엄응용。
Objective To establish a proved spectrophotometric method for the determination of the ischemia-modified albumin( IMA)in serum. Methods The method was established by determinating each component as well as its content in the reagents, exploring the buffered medium, the anti -jamming and antioxidant and so on. Results The method is liner in the range of 0 to 180 U/mL,the average chemical recovery is 99. 3% and a good repeat was obtained with the within - run between 1. 17% to 3. 11% and the between-run 2. 15% to 4. 21%. The regression equations is y=0. 986 7 x+0. 4 with its correlation coefficients being 0. 999 3. Conclusion This method is simple,sensitive and suitable for application.