CAJ | 학술논문

目的：研究白藜芦醇（resveratrol，Res）对人结肠癌细胞增殖的抑制作用及可能的分子机制。方法采用结晶紫染色和 Western blot 分析 Res 对 HCT116细胞增殖的抑制作用；应用流式细胞术和 Western blot 分析 Res 对 HCT116细胞凋亡的促进作用；利用 TCF4／LEF 萤光素酶报告质粒检测Res 对 Wnt／β-catenin 信号转导的影响。采用 Western blot 和PCR 分析 Res 对 HCT116细胞内β-catenin 表达水平的影响。结果与对照组相比，Res 能抑制 HCT116细胞增殖，下调PCNA 蛋白水平；流式细胞术和 Western blot 检测结果均显示，Res 能明显促进 HCT116细胞凋亡；报告质粒分析结果显示，Res 呈浓度依赖性降低TCF4／LEF 报告质粒萤光素酶活性（Res 为20μmol·L －1时，P ＜0.05；Res 为40或80μmol·L －1时，P ＜0．01）；Western blot 和PCR 分析结果显示，Res不仅明显降低HCT116细胞中βcatenin 的蛋白水平，同时也下调βcatenin mRNA 的表达水平。结论 Res 能抑制HCT116细胞增殖并促进凋亡，其机制可能至少与Res 下调βcatenin mRNA 表达，抑制Wnt／βcatenin 信号转导有关。
목적：연구백려호순（resveratrol，Res）대인결장암세포증식적억제작용급가능적분자궤제。방법채용결정자염색화 Western blot 분석 Res 대 HCT116세포증식적억제작용；응용류식세포술화 Western blot 분석 Res 대 HCT116세포조망적촉진작용；이용 TCF4／LEF 형광소매보고질립검측Res 대 Wnt／β-catenin 신호전도적영향。채용 Western blot 화PCR 분석 Res 대 HCT116세포내β-catenin 표체수평적영향。결과여대조조상비，Res 능억제 HCT116세포증식，하조PCNA 단백수평；류식세포술화 Western blot 검측결과균현시，Res 능명현촉진 HCT116세포조망；보고질립분석결과현시，Res 정농도의뢰성강저TCF4／LEF 보고질립형광소매활성（Res 위20μmol·L －1시，P ＜0.05；Res 위40혹80μmol·L －1시，P ＜0．01）；Western blot 화PCR 분석결과현시，Res불부명현강저HCT116세포중βcatenin 적단백수평，동시야하조βcatenin mRNA 적표체수평。결론 Res 능억제HCT116세포증식병촉진조망，기궤제가능지소여Res 하조βcatenin mRNA 표체，억제Wnt／βcatenin 신호전도유관。
Aim To investigate the anti-proliferation effect of resveratrol (Res)on human colon cancer cells and dissect the possible mechanism underlaying this effect.Methods We introduced crystal violet staining and Western blot to analyse the anti-proliferation effect of Res on HCT1 1 6 cells.Then,we used flow cytome-try and Western blot assay to detect the Res induced apoptosis in HCT1 1 6 cells.Next,we employed the well established TCF4 /LEF luciferase reporter to meas-ure the effect of Res on Wnt/β-catenin signaling trans-duction.Finally,we took Western blot and PCR assay to analyse the effect of Res on the expression of β-cate-nin in HCT1 1 6 cells.Results Crystal violet staining and Western blot analysis showed that Res could inhib-it the proliferation of HCT1 1 6 cells in a concentration-and time dependent fashion.What’s more,Res could promote apoptosis in HCT1 1 6 cells.The transcriptional activities of TCF4 /LEF reporter were reduced by Res in a concentration-dependent fashion (P <0.05 when the concentration of Res was 20 μmol·L -1 ,and P <0.01 when the concentration of Res was 40 μmol·L -1 or 80 μmol·L -1 ).Res could decrease not only the protein level of β-catenin in HCT1 1 6 cells,but also the mRNA expression of β-catenin.Conclusion Res can inhibit the proliferation of HCT1 1 6 cells,which may be mediated at least by down-regulating the ex-pression of β-catenin to inhibit the Wnt/β-catenin sig-naling transduction.