中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2015年
4期
527-531
,共5页
汪丽佳%邱红梅%蒋心惠%刘丹%费慧芝%胡小娅%周岐新
汪麗佳%邱紅梅%蔣心惠%劉丹%費慧芝%鬍小婭%週岐新
왕려가%구홍매%장심혜%류단%비혜지%호소아%주기신
抑郁症%慢性不可预见性刺激%色氨酸羟化酶%酪氨酸羟化酶%5-羟色胺%去甲肾上腺素
抑鬱癥%慢性不可預見性刺激%色氨痠羥化酶%酪氨痠羥化酶%5-羥色胺%去甲腎上腺素
억욱증%만성불가예견성자격%색안산간화매%락안산간화매%5-간색알%거갑신상선소
depression%CUS%TPH%TH%5-HT%NE
目的：探讨慢性不可预知性刺激（chronically unpre-dicted stress，CUS）致大鼠抑郁行为与单胺递质合成限速酶色氨酸羟化酶（tryptophan hydroxylase，TPH）和酪氨酸羟化酶（tyrosine hydroxylase，TH）表达的关系。方法♂SD 大鼠30只，随机分为对照组（control group，CG）和模型组（model group，MG），每组15只。采用连续给予 CUS 结合孤养28 d建立抑郁模型；对照组每笼5只，正常饲养；以开场试验和糖水消耗实验进行行为学评价；以荧光定量 PCR 测定海马、前脑皮层 TPH 及 TH mRNA 表达，以 Western blot 法测定海马、前脑皮层 TPH 及 TH 蛋白表达。结果与 CG 相比，MG 大鼠开场实验水平和垂直活动得分均明显降低，糖水消耗量也显著减少，海马、前脑皮层 TPH 及 TH mRNA 和蛋白表达均明显下降。结论CUS 诱导♂大鼠产生抑郁症样行为，其发生机制可能与单胺递质合成限速酶 TPH、TH 表达下调相关。
目的：探討慢性不可預知性刺激（chronically unpre-dicted stress，CUS）緻大鼠抑鬱行為與單胺遞質閤成限速酶色氨痠羥化酶（tryptophan hydroxylase，TPH）和酪氨痠羥化酶（tyrosine hydroxylase，TH）錶達的關繫。方法♂SD 大鼠30隻，隨機分為對照組（control group，CG）和模型組（model group，MG），每組15隻。採用連續給予 CUS 結閤孤養28 d建立抑鬱模型；對照組每籠5隻，正常飼養；以開場試驗和糖水消耗實驗進行行為學評價；以熒光定量 PCR 測定海馬、前腦皮層 TPH 及 TH mRNA 錶達，以 Western blot 法測定海馬、前腦皮層 TPH 及 TH 蛋白錶達。結果與 CG 相比，MG 大鼠開場實驗水平和垂直活動得分均明顯降低，糖水消耗量也顯著減少，海馬、前腦皮層 TPH 及 TH mRNA 和蛋白錶達均明顯下降。結論CUS 誘導♂大鼠產生抑鬱癥樣行為，其髮生機製可能與單胺遞質閤成限速酶 TPH、TH 錶達下調相關。
목적：탐토만성불가예지성자격（chronically unpre-dicted stress，CUS）치대서억욱행위여단알체질합성한속매색안산간화매（tryptophan hydroxylase，TPH）화락안산간화매（tyrosine hydroxylase，TH）표체적관계。방법♂SD 대서30지，수궤분위대조조（control group，CG）화모형조（model group，MG），매조15지。채용련속급여 CUS 결합고양28 d건립억욱모형；대조조매롱5지，정상사양；이개장시험화당수소모실험진행행위학평개；이형광정량 PCR 측정해마、전뇌피층 TPH 급 TH mRNA 표체，이 Western blot 법측정해마、전뇌피층 TPH 급 TH 단백표체。결과여 CG 상비，MG 대서개장실험수평화수직활동득분균명현강저，당수소모량야현저감소，해마、전뇌피층 TPH 급 TH mRNA 화단백표체균명현하강。결론CUS 유도♂대서산생억욱증양행위，기발생궤제가능여단알체질합성한속매 TPH、TH 표체하조상관。
Aim To investigate whether chronically un-predictable stress (CUS)-induced depression-like be-haviors of rats is associated with the variant expression of tryptophan hydroxylase (TPH)and tyrosine hydrox-ylase (TH).Methods 30 male SD rats were ran-domly divided into depression model group(MG)and control group (CG),the former was established using CUS plus solitary condition for 28 d,whereas the latter was fed normally as five rats per cage without CUS. The open field test(OFT)and the sucrose preference test were used to evaluate depressive behaviors.Both mRNA and protein expressions of TPH and TH in hip-pocampus and forebrain cortex were determined by re-al-time fluorescent quantitative PCR and western blot (WB),respectively.Results MG rats showed obvi-ous depressive behaviors with much lower locomotive activity and sucrose preference than CG.Meanwhile, the mRNA and protein expressions of TPH and TH also significantly decreased in MG rats,compared with CG rats.Conclusion The depression behaviors of rats in-duced by CUS may be associated with down-regulation of TPH and TH expression.
