CAJ | 학술논문

目的：观察抗心律失常肽（AAP10）对柯萨奇B3病毒（CVB3）感染的原代小鼠心肌细胞的保护作用。方法将BALB／C小鼠原代心肌细胞分为7组，每组12孔，分别为细胞对照组，病毒对照组，药物干预组（A组、B组、C组、D组、E组）。用不同浓度的AAP10培养液干预100 TCID50 CVB3感染的心肌细胞，对比每组的细胞活性。结果药物干预组细胞活性高于病毒对照组（ F＝7．489，P＜0．05）。其细胞活性与药物浓度（ r＝0．942，P＝0．005）及作用时间（ r＝0．993，P＜0．05）均呈正相关。结论 AAP10具有明显的抗病毒活性，呈剂量时间相关性，对CVB3感染的心肌细胞有保护作用。
목적：관찰항심률실상태（AAP10）대가살기B3병독（CVB3）감염적원대소서심기세포적보호작용。방법장BALB／C소서원대심기세포분위7조，매조12공，분별위세포대조조，병독대조조，약물간예조（A조、B조、C조、D조、E조）。용불동농도적AAP10배양액간예100 TCID50 CVB3감염적심기세포，대비매조적세포활성。결과약물간예조세포활성고우병독대조조（ F＝7．489，P＜0．05）。기세포활성여약물농도（ r＝0．942，P＝0．005）급작용시간（ r＝0．993，P＜0．05）균정정상관。결론 AAP10구유명현적항병독활성，정제량시간상관성，대CVB3감염적심기세포유보호작용。
Objective To observe the anti‐arrhythmic peptide 10(AAP10) against coxsackie virus B3 (CVB3) damage to the generation of myocardial cells in mice .Methods BALB/C mice primary myocardial cells can be divided into 7 groups ,each group of 12 holes :the cell control group ,the virus control group ,and drug intervention group(five different concentrations) .Myocardial cells of 100 TCID50 CVB3 were interventied with different concentrations of AAP10 .Cell damage degree among each group were contrasted .Results Myocardial activity has a positive correla‐tion with the drug concentration (r=0 .942 ,P=0 .005 ,r2 =0 .888) and action time(r=0 .993 ,P<0 .05) .Myocardial activity in intervention group is significantly higher than virus control group(F= 7 .489 ,P< 0 .05) .Conclusion AAP10 has obvious antiviral activity in vitro ,which has a correlation with drug and action time .AAP10 can protect myocardial cell infected by CVB3 in vitro .