CAJ | 학술논문

目的：探讨外源性一氧化碳释放分子2（CORM2）对大鼠脊髓缺血再灌注损伤的保护作用与相关机制。方法将30只健康雄性Wistar大鼠分为实验组、对照组、空白组，每组各10只。实验组在造模前1 h尾静脉注射外源性CORM2。实验组和对照组采用相同的方法造模。对照组造模后1 h注射与实验组相同剂量的生理盐水。空白组仅完成开腹和关腹操作，并术后1h注射与实验组相同剂量的生理盐水。在术后不同时间进行神经行为学评分（Tarlov评分）、脊髓组织病理学检查和白细胞介素‐6（IL‐6）、肿瘤坏死因子‐α（TNF‐α）检测。结果再灌注后不同时间点，实验组Tarlov评分均高于对照组，但实验组和对照组 Tarlov评分均低于空白组（P＜0．05）。缺血再灌注48 h后，对照组脊髓组织标本可见损伤及坏死神经元，表现为细胞核固缩或溶解，细胞质中的尼氏体消失；空白组脊髓组织标本中可见正常神经元，表现为结构呈多角形，细胞质中可见尼氏体；实验组脊髓组织标本中的神经元坏死表现介于对照组和空白组之间。缺血再灌注48 h ，实验组与对照组 IL‐6、T N F‐α表达水平均高于空白组，但实验组IL‐6、TNF‐α表达水平低于对照组（P＜0．05）。结论外源性CORM2可抑制炎性因子的表达，对大鼠脊髓缺血再灌注损伤具有一定的保护作用。
목적：탐토외원성일양화탄석방분자2（CORM2）대대서척수결혈재관주손상적보호작용여상관궤제。방법장30지건강웅성Wistar대서분위실험조、대조조、공백조，매조각10지。실험조재조모전1 h미정맥주사외원성CORM2。실험조화대조조채용상동적방법조모。대조조조모후1 h주사여실험조상동제량적생리염수。공백조부완성개복화관복조작，병술후1h주사여실험조상동제량적생리염수。재술후불동시간진행신경행위학평분（Tarlov평분）、척수조직병이학검사화백세포개소‐6（IL‐6）、종류배사인자‐α（TNF‐α）검측。결과재관주후불동시간점，실험조Tarlov평분균고우대조조，단실험조화대조조 Tarlov평분균저우공백조（P＜0．05）。결혈재관주48 h후，대조조척수조직표본가견손상급배사신경원，표현위세포핵고축혹용해，세포질중적니씨체소실；공백조척수조직표본중가견정상신경원，표현위결구정다각형，세포질중가견니씨체；실험조척수조직표본중적신경원배사표현개우대조조화공백조지간。결혈재관주48 h ，실험조여대조조 IL‐6、T N F‐α표체수평균고우공백조，단실험조IL‐6、TNF‐α표체수평저우대조조（P＜0．05）。결론외원성CORM2가억제염성인자적표체，대대서척수결혈재관주손상구유일정적보호작용。
Objective To investigate the protective effects of exogenous carbon monoxide releasing molecules 2 (CROM2) in rat with spinal cord ischemia and reperfusion injury .Methods A total of 30 healthy male Wistar rats were divided into experiment group ,control group and blank group ,with 10 rats in each group .Rats of experiment group were given exogenous CORM2 by intravenous injection 1 h before modeling .Same operation was performed in experiment group and control group to construct rat models .Rats in control group were injected the same dose of sa‐line 1 h after the construction of models .Rat in blank group were given only abdominal operation without any other treatment ,and were injected the same dose of saline 1 h after operation .At different time point of operation ,evalua‐tion of Tarlov scale ,histopathology examination of myeloid tissue ,and detection of interleukin‐6(IL‐6) and tumor necrosis factor‐α(TNF‐α) were performed .Results At different time point of reperfusion ,Tarlov scores of experi‐ment group were higher than control group ,but those of the two groups were lower than blank group (P<0 .05) .At 48 h after ischemia‐reperfusion ,damage of neuron in control group was obvious ,which was not found in blank group , and the characteristics of neuron damage in experiment group were between control group and blank group .At 48 h after ischemia‐reperfusion ,levels of IL‐6 and TNF‐αin experiment group and control group were significantly higher than blank group ,while those in experiment group were significantly lower than control group (P<0 .05) .Conclusion Exogenous CORM2 could be with protective effects of spinal cord ischemia‐reperfusion injury ,which might be de‐pendent on the inhibition of the releasing of inflammatory cytokines .