CAJ | 학술논문

目的：研究丙型肝炎病毒（HCV ）早期感染者（抗原阳性、抗体阴性）的 HCV 病毒载量与 CD4＋CD25＋叉头／翅膀状螺旋转录因子（Foxp3＋）调节性T淋巴细胞水平的相关性，分析CD4＋ CD25＋ Foxp3＋调节性 T淋巴细胞在 HCV感染中的作用。方法应用实时荧光定量聚合酶链反应（FQ RT‐PCR）技术检测22例 HCV核心抗原阳性、抗体阴性（即 HCV Ag阳性、抗‐HCV阴性）的早期感染病例、26例核心抗原阳性、抗体阳性（即 HCV Ag阳性、抗‐HCV阳性）的慢性 HCV感染病例，24例核心抗原阴性、抗体阳性（即HCV Ag阴性、抗‐HCV阳性）的既往HCV感染病例及22例健康对照组 HCV RNA病毒载量；并用流式细胞技术检测各组外周血有核细胞中的CD4＋CD25＋ Foxp3＋调节性T淋巴细胞水平。结果 HCV Ag阳性、抗‐HCV阴性组，HCV Ag阳性、抗‐HCV阳性组，HCV Ag阴性、抗‐HCV阳性组及健康对照组CD4＋CD25＋ Foxp3＋调节性 T淋巴细胞逐级下降，其中抗原阳性、抗体阴性组[（4．86±2．14）％]高于其他组[分别为（3．74±1．67）％、（2．74±1．27）％、（2．56±1．18）％]，差异有统计学意义（t值分别为2．023、4．125、4．402，P＜0．05）；HCV Ag阳性、抗‐HCV阴性组CD4＋ CD25＋ Foxp3＋调节性T淋巴细胞与HCV RNA病毒载量的对数（copy／mL ，Ig）呈正相关（r＝0．535，P＜0．05）。结论 HCV早期感染者CD4＋CD25＋ Foxp3＋调节性T淋巴细胞水平与高病毒调定点相关，CD4＋CD25＋ Foxp3＋调节性T淋巴细胞水平可能是加速 HCV早期感染的影响因素之一。
목적：연구병형간염병독（HCV ）조기감염자（항원양성、항체음성）적 HCV 병독재량여 CD4＋CD25＋차두／시방상라선전록인자（Foxp3＋）조절성T림파세포수평적상관성，분석CD4＋ CD25＋ Foxp3＋조절성 T림파세포재 HCV감염중적작용。방법응용실시형광정량취합매련반응（FQ RT‐PCR）기술검측22례 HCV핵심항원양성、항체음성（즉 HCV Ag양성、항‐HCV음성）적조기감염병례、26례핵심항원양성、항체양성（즉 HCV Ag양성、항‐HCV양성）적만성 HCV감염병례，24례핵심항원음성、항체양성（즉HCV Ag음성、항‐HCV양성）적기왕HCV감염병례급22례건강대조조 HCV RNA병독재량；병용류식세포기술검측각조외주혈유핵세포중적CD4＋CD25＋ Foxp3＋조절성T림파세포수평。결과 HCV Ag양성、항‐HCV음성조，HCV Ag양성、항‐HCV양성조，HCV Ag음성、항‐HCV양성조급건강대조조CD4＋CD25＋ Foxp3＋조절성 T림파세포축급하강，기중항원양성、항체음성조[（4．86±2．14）％]고우기타조[분별위（3．74±1．67）％、（2．74±1．27）％、（2．56±1．18）％]，차이유통계학의의（t치분별위2．023、4．125、4．402，P＜0．05）；HCV Ag양성、항‐HCV음성조CD4＋ CD25＋ Foxp3＋조절성T림파세포여HCV RNA병독재량적대수（copy／mL ，Ig）정정상관（r＝0．535，P＜0．05）。결론 HCV조기감염자CD4＋CD25＋ Foxp3＋조절성T림파세포수평여고병독조정점상관，CD4＋CD25＋ Foxp3＋조절성T림파세포수평가능시가속 HCV조기감염적영향인소지일。
Objective To study the correlation between HCV viral load in the early HCV‐infected patients(an‐tigen‐positive antibody‐negative) and CD4+ CD25+ Foxp3+ regulatory T cells ,and to know the function of CD4+CD25+ Foxp3+ regulatory T lymphocytes in the HCV infections .Methods We will use the real‐time quantitative polymerase chain reaction(FQ RT‐PCR) technique to detect HCV RNA viral load in 22 cases of early infected pa‐tients with HCV core positive antigen negative antibody(positive HCV Ag ,negative HCV Ab) ,26 cases of chronic HCV‐infected patients with core positive antigen and positive antibody (positive HCV Ag ,positive HCV Ab) ,24 ca‐ses of following HCV‐infected patients with core negative antigen positive antibody (negative HCV Ag ,positive HCV Ab) and 22 cases healthy controls .We also will use flow cytometry technique to detect the level of CD4+ CD25+Foxp3+ regulatory T lymphocytes of nucleated cells in peripheral blood in each group .Results The CD4+ CD25+Foxp3+ regulatory T lymphocytes in the group of positive HCV Ag negative HCV Ab ,the group of positive HCV Ag positive HCV Ab ,the group of negative HCV Ag positive HCV Ab and healthy control group is step‐down ,of which the group of original positive antibody negative antigen (4 .86 ± 2 .14)% is higher than other groups(3 .74 ± 1 .67)% ,(2 .74 ± 1 .27)% ,(2 .56 ± 1 .18)% .The difference is statistically significant(t values were 2 .023 ,4 .125 , 4 .402 ,P<0 .05);CD4+CD25+ Foxp3+ regulatory T lymphocytes and HCV RNA viral load logarithmic (copy/mL , Ig) were positively correlated(r=0 .535 ,P<0 .05) in core positive antigen negative antibody group .Conclusion The level of CD4+CD25+ Foxp3+ regulatory T lymphocytes in HCV early infections is associated with the set‐point of high virus .CD4+CD25+ Foxp3+ regulatory T lymphocytes level may be one of the factors to speed up the early HCV infection .