CAJ | 학술논문

目的：探讨Slit2／Robo4信号通路在神经元迁移中的活力与凋亡影响，为阐明脑缺血后神经血管新生的机制提供理论和实验依据。方法选择成年雄性远交群（SD ）大鼠，建立缺血性脑卒中动物模型，采用Western blot方法测定梗死侧与非梗死侧Slit2和Robo4的表达。取原代培养10～14 d的神经元种六孔板，分为加药组H2 O2（100μmol／L ）、对照组、H2 O2（100μmol／L ）＋Slit2（3μg／mL ）组，行M T T方法检测细胞活力与双染流式细胞术检测细胞凋亡情况。结果 Western blot结果显示，梗死侧Slit2和Robo4表达均较非梗死侧明显升高，差异有统计学意义（P＜0．05）。100μmol／L H2 O2处理原代培养神经元24 h会导致Slit2和Robo4表达升高（P＜0．05）。M T T结果示，H2 O2、H2 O2＋Slit2组细胞活力都有明显下降，其中，H2 O2＋ Slit2组的细胞存活率明显高于单纯H2O2组（P＜0．05）。流式细胞学显示，H2O2＋Slit2组的细胞凋亡率明显低于 H2O2组，差异有统计学意义（P＜0．05）。结论缺血性脑卒中有内源性Slit2／Robo4信号通路的激活，而外源性Slit2可以促进氧化应激时神经元的存活能力，抑制其凋亡作用，从而起到神经保护作用。
목적：탐토Slit2／Robo4신호통로재신경원천이중적활력여조망영향，위천명뇌결혈후신경혈관신생적궤제제공이론화실험의거。방법선택성년웅성원교군（SD ）대서，건립결혈성뇌졸중동물모형，채용Western blot방법측정경사측여비경사측Slit2화Robo4적표체。취원대배양10～14 d적신경원충륙공판，분위가약조H2 O2（100μmol／L ）、대조조、H2 O2（100μmol／L ）＋Slit2（3μg／mL ）조，행M T T방법검측세포활력여쌍염류식세포술검측세포조망정황。결과 Western blot결과현시，경사측Slit2화Robo4표체균교비경사측명현승고，차이유통계학의의（P＜0．05）。100μmol／L H2 O2처리원대배양신경원24 h회도치Slit2화Robo4표체승고（P＜0．05）。M T T결과시，H2 O2、H2 O2＋Slit2조세포활력도유명현하강，기중，H2 O2＋ Slit2조적세포존활솔명현고우단순H2O2조（P＜0．05）。류식세포학현시，H2O2＋Slit2조적세포조망솔명현저우 H2O2조，차이유통계학의의（P＜0．05）。결론결혈성뇌졸중유내원성Slit2／Robo4신호통로적격활，이외원성Slit2가이촉진양화응격시신경원적존활능력，억제기조망작용，종이기도신경보호작용。
Objective To investigate the viability and apoptosis effects of Slit2/Robo4 polarity signaling in neuronal migration for providing theoretical and experimental mechanisms basis for elucidating cerebral ischemia an‐giogenesis .Methods Adult male SD rats were established animal models of ischemic stroke ,the expression of non‐in‐farct infarction side Slit2 and Robo4 were measured by the Western blot method .The neurons cell by 10 -14 days primary cultured were selected and switched to six‐well plat ,there were divided dosing group H2O2 (100 μmol/L) , the control group and H2O2 (100 μmol/L)+ Slit2(3 μg/mL) group ,the cell viability were detected by MTT and double staining apoptosis were detected by flow cytometry .Results Western blot showed infarction side Slit2 and Robo4 expression were significantly higher than that of non‐infarcted side(P<0 .05) .When treated by the 100μmol/L H2O2treatment of primary cultured neurons 24 hours will cause increased expression of Slit2 and Robo4(P<0 .05) .MTT results shown the H2O2 ,H2O2 +Slit2 groups had significantly decreased in cell viability ,which the cell survival rate of H2O2 +Slit2 group were significantly higher than those of H202 group(P<0 .05) .The apoptosis rate of H2O2 +Slit2 group were significantly lower than those of Slit2 H2O2 group(P< 0 .05) .Conclusion Ischemic stroke had Slit2/Robo4 endogenous pathway activated ,and the exogenous Slit2 can promote the viability of the neu‐ronal oxidative stress and inhibit apoptosis ,thereby it can protect the neuro .