CAJ | 학술논문

目的：观察大鼠佐剂性关节炎（adjuvantrheumatoidar-thritis，AA）病程不同阶段的脾脏T细胞亚群及其重要器官的病理学变化，探讨病程不同的阶段AA大鼠T细胞亚群变化的规律及局部组织和全身重要器官的病理学变化特点。方法选用20只♂Lewis大鼠，完全弗氏佐剂（CFA）诱导AA模型，造模后d7、d10、d13、d16、d19、d22进行全身评分和关节炎指数评分，计算关节肿胀数。造模后d14（炎症初期）、d22（炎症高峰期）处死大鼠，流式细胞仪检测炎症初期和炎症高峰期脾脏总T细胞（CD3＋CD4＋）、记忆T细胞（CD4＋CD44＋）、未致敏T细胞（CD4＋CD62L＋）的细胞亚群和Th17（CD4＋IL17＋）细胞亚群比例。d22取心、肝、脾、肺、肾、踝关节、肠系膜淋巴结组织，光镜观察各组织的病理学改变。结果大鼠免疫后10d出现继发侧足爪红肿，炎症初期（d10～d14）和炎症高峰期（d19～d22）AA大鼠的全身评分、关节炎指数评分和关节肿胀数有不同程度的增加。踝关节病理见滑膜组织增生、炎细胞浸润，血管翳出现等，且心、肝、脾、肺、肾、肠系膜淋巴结组织的病理也有不同程度的炎性变化。与正常组比较，炎症初期AA大鼠脾脏总T细胞、记忆T细胞和未致敏T细胞亚群比例都无明显变化、炎症高峰期AA大鼠脾脏总T细胞、记忆T细胞和未致敏T细胞亚群比例明显升高；Th17细胞亚群比例在炎症初期和高峰期都明显升高。结论AA大鼠的T细胞亚群在炎症初期和高峰期出现不同的变化特点；在大鼠AA不仅有关节局部的病理表现，且全身主要脏器组织均有受累。
목적：관찰대서좌제성관절염（adjuvantrheumatoidar-thritis，AA）병정불동계단적비장T세포아군급기중요기관적병이학변화，탐토병정불동적계단AA대서T세포아군변화적규률급국부조직화전신중요기관적병이학변화특점。방법선용20지♂Lewis대서，완전불씨좌제（CFA）유도AA모형，조모후d7、d10、d13、d16、d19、d22진행전신평분화관절염지수평분，계산관절종창수。조모후d14（염증초기）、d22（염증고봉기）처사대서，류식세포의검측염증초기화염증고봉기비장총T세포（CD3＋CD4＋）、기억T세포（CD4＋CD44＋）、미치민T세포（CD4＋CD62L＋）적세포아군화Th17（CD4＋IL17＋）세포아군비례。d22취심、간、비、폐、신、과관절、장계막림파결조직，광경관찰각조직적병이학개변。결과대서면역후10d출현계발측족조홍종，염증초기（d10～d14）화염증고봉기（d19～d22）AA대서적전신평분、관절염지수평분화관절종창수유불동정도적증가。과관절병리견활막조직증생、염세포침윤，혈관예출현등，차심、간、비、폐、신、장계막림파결조직적병리야유불동정도적염성변화。여정상조비교，염증초기AA대서비장총T세포、기억T세포화미치민T세포아군비례도무명현변화、염증고봉기AA대서비장총T세포、기억T세포화미치민T세포아군비례명현승고；Th17세포아군비례재염증초기화고봉기도명현승고。결론AA대서적T세포아군재염증초기화고봉기출현불동적변화특점；재대서AA불부유관절국부적병리표현，차전신주요장기조직균유수루。
Aim To observe the variation of T-cell subsets in spleen in different courses of disease and tis-sue histopathological changes in rats with adjuvant ar-thritis (AA).Methods The model of rat AA was in-duced by complete Freund’s adjuvant into the right hind metatarsal footpad of 20 male Lewis rats.Arthritis was evaluated by total score,arthritis index and paw swelling number. The percentage of total (CD3 +CD4 +), memory (CD4 + CD44 +), no-sensitizated (CD4 + CD62L +) and Th1 7 (CD4 + IL-1 7 +) on CD4 +T cells in spleen were detected by flow cytometry on days 1 4 and 22 after immuniozation.The his-topathological evaluation of heart,liver,spleen,lung, kidney,ankle joints and mesenteric lymph nodes were examined by hematoxylin and eosin (HE)stain.Re-sults The onset of secondary arthritis appeared on a-bout day 1 0 after immunization,with a peak onset on day 1 9.Systematic arthritis symptoms included front and hind paw swelling and redness (swelling was more apparent in the front rather than hind paws),nodule formation and redness on the ears,connective tissue swelling and redness of the nose and evident nodules and redness on the tail.Total score,arthritis index and the paw swelling numbers were enhanced in AA rats. Pathohistology of ankles joints showed hyperplasia, pannus formation,and inflammatory cells infiltration in AA rats.And pathohistology of heart,liver,spleen, kidney and mesenteric lymph nodes showed inflamma-tory changes.Compared with normal group,there was no change in the percentage of total,memory and no-sensitizated CD4 +T cell in the early stage inflammation but they were significantly enhanced in the peak of in-flammation.Th1 7 cell subsets were significantly en-hanced in both of the early and peak of inflammation. Conclusion These findings suggest that abnormal T cell responses and tissue histopathological changes are important features of AA rats.