CAJ | 학술논문

木质素降解物是影响木质纤维素水解液乙醇发酵效率的主要原因之一。为探明木质素降解物对酿酒酵母的毒性，通过酿酒酵母（ Saccharomyces cerevisiae） CGMCC 2?1429的液体培养，测定木质素制剂及酚类化合物（香草醛、紫丁香醇和对羟基苯甲醛）对细胞的毒性，同时分析酚类化合物的联合作用类型，最后以果糖1，6二磷酸（ FDP ）为指标来分析木质素制剂及酚类化合物酵母细胞膜通透性的变化。结果表明：木质素制剂、紫丁香醇、对羟基苯甲醛和香草醛的半致死质量浓度分别为10?96、5?37、6?17和7?08 g／L。随着各酚类化合物浓度和种类的增加，它们对细胞的毒性随之增加，呈正协同作用。在培养液中，FDP浓度随各酚类化合物浓度的增加呈线性增加，当各酚类化合物的质量浓度从1?58 g／L增加到10?00 g／L时，FDP质量浓度从49?7 mg／L增加到134?4 mg／L。木质素降解物对酿酒酵母的毒性作用与细胞膜通透性的改变密切相关。
목질소강해물시영향목질섬유소수해액을순발효효솔적주요원인지일。위탐명목질소강해물대양주효모적독성，통과양주효모（ Saccharomyces cerevisiae） CGMCC 2?1429적액체배양，측정목질소제제급분류화합물（향초철、자정향순화대간기분갑철）대세포적독성，동시분석분류화합물적연합작용류형，최후이과당1，6이린산（ FDP ）위지표래분석목질소제제급분류화합물효모세포막통투성적변화。결과표명：목질소제제、자정향순、대간기분갑철화향초철적반치사질량농도분별위10?96、5?37、6?17화7?08 g／L。수착각분류화합물농도화충류적증가，타문대세포적독성수지증가，정정협동작용。재배양액중，FDP농도수각분류화합물농도적증가정선성증가，당각분류화합물적질량농도종1?58 g／L증가도10?00 g／L시，FDP질량농도종49?7 mg／L증가도134?4 mg／L。목질소강해물대양주효모적독성작용여세포막통투성적개변밀절상관。
Lignin degradation products are main inhibitors that restrain the ethanol production by fermentation of lignocellulosic hydrolysate. The objective of the present study was to clarify the inhibition of lignin degradation products on Saccharomyces cerevisiae. The toxicity of lignin preparation and phenolic compounds such as vanilla aldehyde,lilac alcohol,and p?hydroxy benzaldehyde was measured by culturing Saccharomyces cerevisiae CGMCC 2?1429. Then,the change of cell membrane permeability was evaluated by the concentration of fructose?1,6?diphosphate ( FDP ) . Results show that the 50% lethal concentration ( LC50 ) of lignin preparation, vanilla aldehyde, lilac alcohol, and p?hydroxy benzaldehyde was 10?96, 5?37,6?17 and 7?08 g/L,respectively. All the phenolic compounds acted synergistically on yeast cells. Fructose?1,6?diphosphate ( FDP ) concentration in culture medium increased linearly with increasing logarithmic concentration of each phenolic compound. When the concentration of each phenolic compound increased from 1?58 g/L to 10?00 g/L,FDP concentration increased from 49?7 mg/L to 134?4 mg/L. The toxicity of lignin degradation products on Saccharomyces cerevisiae closely related to the change of cell membrane permeability.