中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2015年
4期
518-523
,共6页
吴县%江勤%庞刚%刘欢%陶欣荣%董六一%章功良
吳縣%江勤%龐剛%劉歡%陶訢榮%董六一%章功良
오현%강근%방강%류환%도흔영%동륙일%장공량
5-羟色胺%5-HT2C 受体%吗啡%依赖%纳洛酮%戒断症状
5-羥色胺%5-HT2C 受體%嗎啡%依賴%納洛酮%戒斷癥狀
5-간색알%5-HT2C 수체%마배%의뢰%납락동%계단증상
serotonin%5-HT2C receptor%morphine%dependent%naloxone%withdrawal symptoms
目的:观察5-羟色胺2C 受体(5-HT2C R)激动剂 WAY对吗啡依赖小鼠纳洛酮诱导戒断症状的防治作用,探讨5-HT2C R 在吗啡成瘾导致的躯体依赖中的作用。方法采用EthoVision 动物行为分析系统观察5-HT2C R 激动剂 WAY 对正常小鼠自发活动的影响;剂量递增法诱导小鼠吗啡依赖模型,观察 WAY 对吗啡依赖小鼠纳洛酮诱导戒断症状的影响。结果WAY (0.5、0.75、1.0 mg·kg -1,i.p.)对小鼠自发活动没有明显影响。慢性吗啡诱导依赖小鼠运动距离、速度和跳跃等行为活动增加。WAY (0.5、0.75、1.0 mg· kg -1)和可乐定(0.2 mg·kg -1,i.p.)对吗啡依赖小鼠纳洛酮催促出现的打洞、跳跃、清理皮毛、站立、“湿狗”样抖动、
目的:觀察5-羥色胺2C 受體(5-HT2C R)激動劑 WAY對嗎啡依賴小鼠納洛酮誘導戒斷癥狀的防治作用,探討5-HT2C R 在嗎啡成癮導緻的軀體依賴中的作用。方法採用EthoVision 動物行為分析繫統觀察5-HT2C R 激動劑 WAY 對正常小鼠自髮活動的影響;劑量遞增法誘導小鼠嗎啡依賴模型,觀察 WAY 對嗎啡依賴小鼠納洛酮誘導戒斷癥狀的影響。結果WAY (0.5、0.75、1.0 mg·kg -1,i.p.)對小鼠自髮活動沒有明顯影響。慢性嗎啡誘導依賴小鼠運動距離、速度和跳躍等行為活動增加。WAY (0.5、0.75、1.0 mg· kg -1)和可樂定(0.2 mg·kg -1,i.p.)對嗎啡依賴小鼠納洛酮催促齣現的打洞、跳躍、清理皮毛、站立、“濕狗”樣抖動、
목적:관찰5-간색알2C 수체(5-HT2C R)격동제 WAY대마배의뢰소서납락동유도계단증상적방치작용,탐토5-HT2C R 재마배성은도치적구체의뢰중적작용。방법채용EthoVision 동물행위분석계통관찰5-HT2C R 격동제 WAY 대정상소서자발활동적영향;제량체증법유도소서마배의뢰모형,관찰 WAY 대마배의뢰소서납락동유도계단증상적영향。결과WAY (0.5、0.75、1.0 mg·kg -1,i.p.)대소서자발활동몰유명현영향。만성마배유도의뢰소서운동거리、속도화도약등행위활동증가。WAY (0.5、0.75、1.0 mg· kg -1)화가악정(0.2 mg·kg -1,i.p.)대마배의뢰소서납락동최촉출현적타동、도약、청리피모、참립、“습구”양두동、
Aim To explore the effect of pharmacolog-ical activation of serotonin 5-HT2C receptor (5-HT2C R) on naloxone-precipitated withdrawal in morphine-de-pendent mice. Method EthoVision Noldus video tracking system was used to record the effect of 5-HT2C R agonist WAY on locomotor activities and behavioral performances in mice.Results Selective 5-HT2C R ag-onist WAY (0.5,0.75 or 1 .0 mg·kg -1 ,i.p.)a-lone did not alter the locomotor activities as determined by distance traveled and velocity (all P values >0.05).Chronic morphine treatment induced depend-ence in mice as demonstrated by increases in distance traveled,velocity and jumping behavior.WAY (0.5, 0.75 or 1 .0 mg·kg -1 ,i.p.)and clonidine (0.2 mg ·kg -1 ,i.p.)significantly ameliorated naloxone-pre-cipitated withdrawal symptoms,including burrowing, jumping,body grooming,rearing,“wet dog”shakes, head shakes,face grooming,penile grooming,scratch (all P values <0.05).Conclusion Pharmacological activation of 5-HT2C R ameliorates naloxone-precipitated withdrawal symptoms in morphine-dependent mice.5-HT2C R may be a novel target to develop therapeutic ap-proach against morphine physical dependence,craving and relapsing.