CAJ | 학술논문

目的：观察大鼠戊四唑急性癫痫模型造模后不同时间海马神经元损伤程度的变化。方法大鼠腹腔注射10 g· L －1（64 mg·kg －1）戊四唑1次，诱发大鼠急性癫痫发作，分别于注射戊四唑后24、72、120、144 h 将大鼠麻醉，灌流取脑，采用尼氏染色及免疫组化染色观察大鼠海马神经元损伤程度。结果与空白对照组相比，腹腔注射戊四唑后海马神经元损伤程度随着时间的延长逐渐加重。结论戊四唑急性致痫模型大鼠海马神经元损伤的最大差异出现在腹腔注射戊四唑后120 h 附近，可以将其作为药效学研究的海马组织取材时间。
목적：관찰대서무사서급성전간모형조모후불동시간해마신경원손상정도적변화。방법대서복강주사10 g· L －1（64 mg·kg －1）무사서1차，유발대서급성전간발작，분별우주사무사서후24、72、120、144 h 장대서마취，관류취뇌，채용니씨염색급면역조화염색관찰대서해마신경원손상정도。결과여공백대조조상비，복강주사무사서후해마신경원손상정도수착시간적연장축점가중。결론무사서급성치간모형대서해마신경원손상적최대차이출현재복강주사무사서후120 h 부근，가이장기작위약효학연구적해마조직취재시간。
Aim To observe histopathological changes of hippocampus after acute epilepsy induced by penty-lenetetrazole (PTZ)in rats.Methods Five groups as control group,PTZ-induced 24 hours(h)group,PTZ-induced 72 hours group,PTZ-induced 1 20 hours group and PTZ-induced 1 44 hours group were designed.PTZ (64 mg·kg -1 )was administered with a single intrap-eritoneal injection for generalized tonic-clonic sei-zures in the current experiment.Control and PTZ trea-ted animals were sacrificed after specific time points. Brain was dissected out and then evaluated for neuro-pathological changes using Nissl staining and immuno-histochemical technique.Results In this study PTZ-induced hippocampal neuron status apoptosis occurred at 24 hours and was sustained for 1 44 hours after status epilepticus.Whereas,activated caspase-3 and AIF ap-peared at 24 hours and were sustained for 1 44 hours af-ter status epilepticus.Conclusion The results of this study show that the significant histopathological chan-ges of hippocampus appear in the vicinity of 1 20 hours after intraperitoneal injection of pentylenetetrazole.