CAJ | 학술논문

目的：探讨河北地区受激活调节正常 T 细胞表达和分泌因子（regulated upon activation normal T cell ex－pressed and secreted factor，RANTES）基因－403G／A 和－28C／G 单核苷酸多态性（SNP）与结核性脓胸的关系。方法采用聚合酶链反应－限制性片段长度多态性（PCR－RFLP）方法检测300例结核性脓胸患者和300例对照人群RANTES基因－403G／A 和－28C／G 的基因型。结果 RANTES 基因－403G／A SNP 基因型频率和等位基因频率在结核性脓胸组和正常组间差异有统计学意义（ P ＝0．004和 P ＝0．008），与 GG 基因型相比，携带 AG ＋AA 基因型可增加结核性脓胸发病风险（OR ＝1．40395％，CI ＝1．015～1．939）。 RANTES 基因－28C／G SNP 基因型频率和等位基因频率在结核性脓胸组和正常组间差异无统计学意义（ P ＝0．128和 P ＝0．064），与 CC 基因型相比，携带 CG ＋GG 基因型与结核性脓胸发病风险无关（OR ＝1．31495％，CI ＝0．891～1．936）。结论 RANTES 基因－403G／A SNP 可能与结核性脓胸的发病风险有关，即携带 AG ＋AA 基因型增加结核性脓胸发病风险；RANTES 基因－28C／G SNP 可能与结核性脓胸发病风险无关。
목적：탐토하북지구수격활조절정상 T 세포표체화분비인자（regulated upon activation normal T cell ex－pressed and secreted factor，RANTES）기인－403G／A 화－28C／G 단핵감산다태성（SNP）여결핵성농흉적관계。방법채용취합매련반응－한제성편단장도다태성（PCR－RFLP）방법검측300례결핵성농흉환자화300례대조인군RANTES기인－403G／A 화－28C／G 적기인형。결과 RANTES 기인－403G／A SNP 기인형빈솔화등위기인빈솔재결핵성농흉조화정상조간차이유통계학의의（ P ＝0．004화 P ＝0．008），여 GG 기인형상비，휴대 AG ＋AA 기인형가증가결핵성농흉발병풍험（OR ＝1．40395％，CI ＝1．015～1．939）。 RANTES 기인－28C／G SNP 기인형빈솔화등위기인빈솔재결핵성농흉조화정상조간차이무통계학의의（ P ＝0．128화 P ＝0．064），여 CC 기인형상비，휴대 CG ＋GG 기인형여결핵성농흉발병풍험무관（OR ＝1．31495％，CI ＝0．891～1．936）。결론 RANTES 기인－403G／A SNP 가능여결핵성농흉적발병풍험유관，즉휴대 AG ＋AA 기인형증가결핵성농흉발병풍험；RANTES 기인－28C／G SNP 가능여결핵성농흉발병풍험무관。
Objective To investigate the correlation between RANTES-403G/A and-28C/G single nucleotide polymorphism (SNPs) and the onset risk of tuberculous empyema (TE) in Hebei Province,China.Methods The gene types of SNPs of RANTES-403G/A and-28C/G were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 300 patients with tuberculous empyema (patient group) and 300 healthy subjects (control group).Results There were significant differences in genotype frequency and allele frequency of RANTES-403G /A SNP between the two groups (P =0.004,0.008,respectively).As compared with GG genotype,the AG +GG genotype could increase the onset risk of tuberculous empyema (OR =1.403 95%,CI =1.015 ~1.939).However there were no significant differences in genotype frequency and allele frequency of RANTES-28C/G between the patient group and control group ( P =0.128, 0.064, respectively).As compared with the CC genotype,the CG +GG genotype was not correlated to onset risk of TE (OR =1.314 95%,CI =0.891 ~1.936).Conclusion The RANTES-403G/A SNP may be correlated to onset risk of TE,carrying G allele (AG +GG) may increase onset risk of TE.However 28C/G SNP may be not related with onset risk of TE.