CAJ | 학술논문

目的：检测 Caspase－3及 Survivin 在急性胰腺炎大鼠胰腺组织中的表达并探讨其与胰腺组织损伤的关系。方法60只 SPF 级雄性 Wistar 大鼠随机分为假手术组、胰腺炎6 h 组、胰腺炎12 h 组及胰腺炎24 h 组。采用胰腺被膜下注射牛磺胆酸钠（Na－Tc）建立急性胰腺炎模型。分别检测4组大鼠血清淀粉酶（AMY）、胰腺病理损伤、胰腺组织中 Caspase－3及 Survivin 的表达程度。结果假手术组、胰腺炎6 h、12 h、24 h 组组间两两比较血清淀粉酶及胰腺组织病理评分胰腺组织 Caspase－3及 Survivin 平均光密度表达差异均有统计学意义（ P ＜0．05）。 Caspase－3、Survivin、血清淀粉酶及胰腺病理评分均有相关性（ P ＜0．05）。结论急性胰腺炎过程中伴随着 Caspase－3及 Survivin 的表达紊乱，并且与胰腺损伤密切相关。
목적：검측 Caspase－3급 Survivin 재급성이선염대서이선조직중적표체병탐토기여이선조직손상적관계。방법60지 SPF 급웅성 Wistar 대서수궤분위가수술조、이선염6 h 조、이선염12 h 조급이선염24 h 조。채용이선피막하주사우광담산납（Na－Tc）건립급성이선염모형。분별검측4조대서혈청정분매（AMY）、이선병리손상、이선조직중 Caspase－3급 Survivin 적표체정도。결과가수술조、이선염6 h、12 h、24 h 조조간량량비교혈청정분매급이선조직병리평분이선조직 Caspase－3급 Survivin 평균광밀도표체차이균유통계학의의（ P ＜0．05）。 Caspase－3、Survivin、혈청정분매급이선병리평분균유상관성（ P ＜0．05）。결론급성이선염과정중반수착 Caspase－3급 Survivin 적표체문란，병차여이선손상밀절상관。
Objective To detect the expression levels of Caspase-3 and Survivin in rats with acute pancreatitis,and to explore their correlation with pancreatic tissue damage.Methods Sixty SPF male Wistar rats were randomly divided into four groups:sham-operation group ( n =15),6 hours after pancreatitis group (6h group, n =15),12 hours after pancreatitis group (12h group, n =15),24 hours after pancreatitis group (24h group, n =15).The animal models with pancreatitis were induced by injection of 5% Na-Tc under capsula pancreatis.The activity of serum amylase,pathological injury of pancreas, expression levels of Caspase-3 and Survivin were detected for all the groups.Results There were significant differences in the activity of serum amylase, pathological injury of pancreas and the expression levels of Caspase-3 and Survivin among sham-operation group,6h group,12h group,24h group with each other ( P <0.05).There were close correlations among Caspase-3,Survivin, serum amylase and pathological injury scoring.Conclusion The process of acute pancreatitis is accompanied with the expression disorder of Caspase-3 and Survivin, furthermore, which is closely correlated to pancreas injury.