CAJ | 학술논문

通过诱变选育，将铜绿假单胞菌（ Pseudomonas aeruginosa） RG 14利用甘油发酵生产鼠李糖脂产量由13?6 g／L提高到16?5 g／L。突变株经过5次连续传代培养，菌株仍维持稳定的鼠李糖脂产量，表明该菌株具有较好的遗传稳定性。利用基质辅助激光解析电离飞行时间质谱（ MALDI TOF MS）技术分析诱变后菌株发酵甘油生产鼠李糖脂的组成，结果显示鼠李糖脂由Rha C8 C8、Rha C8 C10、Rha C10 C10、Rha C10 C12∶1、Rha C10 C12、Rha2 C8 C10、Rha2 C10 C10、Rha2 C10 C12∶1和Rha2 C10 C12组成，其中单、双鼠李糖脂的相对丰度分别为54?8％和45?2％。当以工业粗甘油代替精甘油为底物时，该菌株鼠李糖脂产量达到14?2 g／L，表明其具有较好的应用潜力。
통과유변선육，장동록가단포균（ Pseudomonas aeruginosa） RG 14이용감유발효생산서리당지산량유13?6 g／L제고도16?5 g／L。돌변주경과5차련속전대배양，균주잉유지은정적서리당지산량，표명해균주구유교호적유전은정성。이용기질보조격광해석전리비행시간질보（ MALDI TOF MS）기술분석유변후균주발효감유생산서리당지적조성，결과현시서리당지유Rha C8 C8、Rha C8 C10、Rha C10 C10、Rha C10 C12∶1、Rha C10 C12、Rha2 C8 C10、Rha2 C10 C10、Rha2 C10 C12∶1화Rha2 C10 C12조성，기중단、쌍서리당지적상대봉도분별위54?8％화45?2％。당이공업조감유대체정감유위저물시，해균주서리당지산량체도14?2 g／L，표명기구유교호적응용잠력。
Through mutation, rhamnolipids yield of Pseudomonas aeruginosa RG?14 increased from 13?6 g/L to 16?5 g/L. After 5 rounds of subculturing, rhamnolipids yield of the mutant stayed stable. Subsequently, MALDI?TOF?MS was applied to analyze the composition of rhamnolipids produced by the mutant. The results showed that the rhamnolipids congener was comprised of Rha?C8?C8,Rha?C8?C10, Rha?C10?C10, Rha?C10?C12 ∶ 1, Rha?C10?C12, Rha2?C8?C10, Rha2?C10?C10, Rha2?C10?C12 ∶ 1 and Rha2?C10?C12. The relative abundance of mono?rhamnolipids and di?rhamnolipids were 54?8% and 45?2%, respectively. The mutant strain also could use crude glycerol besides refined glycerol, and the rhamnolipids yield reached 14?2 g/L, showing application potential of this strain.