CAJ | 학술논문

为了提高黑曲霉（ Aspergillus niger） D08生产木聚糖酶的能力，考察了不同pH条件对菌株生长及产酶的影响，结果发现木聚糖酶发酵过程中菌体生长的最适pH与木聚糖酶合成的最适pH不同，分别为3?5和4?5。由此针对性地提出了两阶段pH控制策略：在30 h内控制pH为3?5；30 h后pH控制为4?5。结果表明：分阶段pH调控策略的实施进一步提高了菌体合成木聚糖酶的能力，木聚糖酶比酶活（2223?38 U／mL ）和生产强度（26?47 U／（ mL·h））分别比恒定pH 4?5时提高了14?3％和30?6％。该工艺对提高木聚糖酶活力具有一定的指导意义，为后续研究和生产奠定了良好的基础。
위료제고흑곡매（ Aspergillus niger） D08생산목취당매적능력，고찰료불동pH조건대균주생장급산매적영향，결과발현목취당매발효과정중균체생장적최괄pH여목취당매합성적최괄pH불동，분별위3?5화4?5。유차침대성지제출료량계단pH공제책략：재30 h내공제pH위3?5；30 h후pH공제위4?5。결과표명：분계단pH조공책략적실시진일보제고료균체합성목취당매적능력，목취당매비매활（2223?38 U／mL ）화생산강도（26?47 U／（ mL·h））분별비항정pH 4?5시제고료14?3％화30?6％。해공예대제고목취당매활력구유일정적지도의의，위후속연구화생산전정료량호적기출。
In order to improve xylanase production of Aspergillus niger D08,effects of pH on cell growth and xylanase production were investigated. The optimum pH for cell growth was 3?5, while that for xylanase production was 4?5. Accordingly, a two?stage pH control strategy was as follows:pH was controlled at 3?5 for the first 30 h,and then raised to 4?5 for the remaining time. With this strategy,the maximal xylanase activity and productivity of xylanase reached 2 223?38 U/mL and 26?47 U/( mL·h) ,respectively,which were 14?3% and 30?6% higher than those in the single pH ( pH 4?5) controlled model. This pH control strategy offers a promising guideline for enhancing the production of xylanase by Aspergillus niger D08,thus making a good foundation to further research and production of xylanase.