山西中医学院学报
山西中醫學院學報
산서중의학원학보
JOURNAL OF SHANXI COLLEGE OF TRADITIONAL CHINESE MEDICINE
2014年
6期
30-31,37
,共3页
地龙%溶栓胶囊%口蹄疫病毒%RT-PCR%病毒测定
地龍%溶栓膠囊%口蹄疫病毒%RT-PCR%病毒測定
지룡%용전효낭%구제역병독%RT-PCR%병독측정
Pheretima asiatica%Rongshuan capsule%foot-and-mouth disease virus%RT-PCR%virus detection
目的：用RT-PCR法检测地龙及其制剂溶栓胶囊中是否存有口蹄疫病毒。方法：提取样品总RNA，用一步法RT-PCR扩增，扩增产物经琼脂糖凝胶电泳后，以电泳图中待测样品扩增产物是否出现与阳性对照相同的条带来判断样品是否为阳性。结果：电泳结果显示，不同效期3批溶栓胶囊和地龙冻干粉原料口蹄疫病毒检测结果均为阴性，提示其不含有口蹄疫病毒。结论：该法简便、快捷，具较高的特异性和灵敏性，可以为中药材及制剂中病毒的检测提供参考。
目的：用RT-PCR法檢測地龍及其製劑溶栓膠囊中是否存有口蹄疫病毒。方法：提取樣品總RNA，用一步法RT-PCR擴增，擴增產物經瓊脂糖凝膠電泳後，以電泳圖中待測樣品擴增產物是否齣現與暘性對照相同的條帶來判斷樣品是否為暘性。結果：電泳結果顯示，不同效期3批溶栓膠囊和地龍凍榦粉原料口蹄疫病毒檢測結果均為陰性，提示其不含有口蹄疫病毒。結論：該法簡便、快捷，具較高的特異性和靈敏性，可以為中藥材及製劑中病毒的檢測提供參攷。
목적：용RT-PCR법검측지룡급기제제용전효낭중시부존유구제역병독。방법：제취양품총RNA，용일보법RT-PCR확증，확증산물경경지당응효전영후，이전영도중대측양품확증산물시부출현여양성대조상동적조대래판단양품시부위양성。결과：전영결과현시，불동효기3비용전효낭화지룡동간분원료구제역병독검측결과균위음성，제시기불함유구제역병독。결론：해법간편、쾌첩，구교고적특이성화령민성，가이위중약재급제제중병독적검측제공삼고。
Objective:To detect foot-and-mouth disease virus in Pheretima asiatica freeze dried powder and its prepara-tions (Rongshuan capsule)by RT-PCR method. Method:Total RNA of samples was extracted and was amplified by one-step RT-PCR method. Then electrophoregram of amplification product of samples was detected. Result:It showed that foot-and-mouth disease virus determination results of 3 batches of Rongshuan capsule samples and raw material of Phere-tima asiatica were negative. Conclusion:One-step RT-PCR method is simple,rapid,specific and sensitive and could be provided as a reference for detection of virus in Chinese crude drug and preparations.
